FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1248777
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC36040.2886023685573965No Hit
GTAATAATCAGCCTCATCCTCAGGCTGGAGCCCAGAGATGGTCAGGGAGG26230.2100455085255414No Hit
GATTATTACTGCTGCTCATTTGCAGGCGACAACACTTGGGTGTTCGGCGG25060.2006763417327513No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTGTCG23330.18682278741520705No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20550.16456100648874858No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA19920.15951607052340008No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19010.15222894079567448No Hit
TCTCTGGGCTCCAGCCTGAGGATGAGGCTGATTATTACTGCTGCTCATTT17980.14398087088407296No Hit
CTCCTGGACAGTCAGCCACCATCTCCTGCACTGGAACCAGCAGTGATGTT17870.1431000090488534No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT17370.13909609161603712No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17290.13845546482678653No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC16380.13116833509906092No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA15730.12596324243639978No Hit
GTGTCCGGGTCTCCTGGACAGTCAGCCACCATCTCCTGCACTGGAACCAG15470.12388120537133532No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTTTGTCTCCT14290.11443196022988891No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13950.11170929637557388No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13850.11090851288901062No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13800.11050812114572897No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA13750.11010772940244735No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13700.10970733765916572No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTGTCGC12740.10201981618815849No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA12700.1016995027935332No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22300.041.682061
ATCAACG21850.039.8639833
TCAACGC22250.038.8237954
CAACGCA23800.036.5978165
TATCAAC25500.035.0048562
AACGCAG25350.034.3600776
CGCAGAG28250.030.5780328
TATACCG1000.00628998128.7943135
GAATACG3351.8189894E-1227.9347829
ACGCAGA32050.027.4017687
GTACATG38050.026.5117051
TACATGG36350.025.7445722
GCAGAGT34600.024.966179
ACAGCCG9250.024.903198
ACATGGG38450.024.3384973
TAGACAG13050.024.271075
TAGCGCG1500.001557722223.9952624
TATTGAG17900.023.3250035
ACCGACC3503.803507E-922.6241048
CAGCCGT9500.021.9746079
Produced by FastQC (version 0.11.5)