FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116007
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGATACCACTGCTTGATACCACTGCTTGATACCACTGCTTGATACCA21190.18987336100938437No Hit
GTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAG20680.18530349720028638No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15530.1391568332456696No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA14380.12885223838201731No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT12020.10770541761834827No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11350.10170187104561174No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC36200.043.5537574
GAATACG5400.042.66259
CAACGCA37550.041.796185
AACGCAG38450.040.8178566
TATCAAC40500.039.3432242
ACGCAGA40050.039.0074277
CGCAGAG40700.038.3844578
ATCAACG44900.035.274953
TAGTACG1459.190553E-734.7552264
GACCGTT850.00283810433.8790448
GTGCTAG8150.032.7911721
GCAGAGT47950.032.73099
GTGTAGA7200.030.0954991
TGCACCG3500.028.7971865
CTAGAGA10000.028.0772574
ACCGACC2604.0108716E-927.68968
TCTATAC4200.025.7117753
AGTACGG1400.0010413125.7117735
TCTACAC12950.025.5727923
GTACGGA1450.001277745424.8251636
Produced by FastQC (version 0.11.5)