FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences798493
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG34540.43256484402493195No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG32690.4093962000919231No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT24650.3087065259181984No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG24500.3068279872209274No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT20750.2598645197891528No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG20280.2539784318710371No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC19360.24245672786110836No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC19120.2394510659454748No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT16490.20651402078665687No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT14950.18722769016134144No Hit
GTATACAGCCGTGTCCTCGGCTCTCAGTCTGTTCATTTGCAGATACAGCG14850.18597533102982744No Hit
GATTAAGACTGATGGGAGTGTAACAAGTTACGCGGACTCCGTGAAGGGCC14470.18121636633007426No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCAAGTGTTCCTG13130.1644347539677868No Hit
GCTGTATACTTCTGCGCACGAGATCTCAGGGGATCCAACTACTGGGGCCA12660.15854866604967105No Hit
GAAGTATACAGCCGTGTCCTCGGCTCTCAGTCTGTTCATTTGCAGATACA12500.15654489143924868No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC12500.15654489143924868No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG12340.15454111682882632No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC12200.15278781404470673No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG11940.14953168030277034No Hit
CCCTGAGGAGACGGCGACCAGGGTTCCCTGGCCCCAGTAGTTGGATCCCC11930.14940644438961895No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT11800.14777837751865075No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC11360.1422679973399892No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC11170.13988851499011262No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG10680.13375195524569408No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAAAGAAGTT10580.1324995961141801No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10510.1316229447221203No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC10410.1303705855906063No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC10240.12824157506703251No Hit
GTCTTAATCTGTGAGACCCATTCCGGCCCCTTCCCTGGAGGTTGGCGGAT10200.12774063141442693No Hit
GGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTG10140.12698921593551854No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCA10130.12686398002236715No Hit
GACCCACACAGACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCA9970.12486020541194476No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT9960.12473496949879335No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCG9740.12197977940946257No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9590.12010124071219158No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCCGGGGGAGGCT9550.119600297059586No Hit
GTATACTTCTGCGCACGAGATCTCAGGGGATCCAACTACTGGGGCCAGGG9510.1190993534069804No Hit
GTGGATGCAGAGGGGGCAGCCCTTGTCCCCGGAGAAGTATGTGACCAGCG9440.11822270201492059No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT9310.11659463514395241No Hit
GATTATTACTGCAGCTCATCTACAAACAGGAACACTTGGGTTTTCGGCGG9300.11646939923080102No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGC9250.11584321966504403No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA9060.11346373731516744No Hit
CTCCTGCACTGGAACCAGCAAAGAAGTTGGTGGTTATGACTATGTCTCCT9050.11333850140201604No Hit
ACACAGACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGG9020.11296279366256184No Hit
GTGTGAGGTGCAGCTGGTGGAGTCCGGGGGAGGCTTAGTTCAGCCTGGGG8900.11145996270474506No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCT8850.11083378313898806No Hit
CTCCTGTGCAGCCTCTGGATTCATCTTCGGTGACTACTGGATGCACTGGA8800.11020760357323107No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8700.10895524444171707No Hit
CTCCAGAGACAACGCCAAGAACACGCTGTATCTGCAAATGAACAGACTGA8640.1082038289628087No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA8630.10807859304965729No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC8620.10795335713650589No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCATCTTCGGTGACTACTGG8590.1075776493970517No Hit
CACGTGCACCGTGACCCACACAGACCTGCCCTCGCCACTGAAGCAGACCA8540.10695146983129471No Hit
CTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCCCGTCACCAGGC8520.10670099800499191No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCAAGTGTTCCTGT8470.10607481843923491No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCA8220.10294392061044993No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA8150.10206726921839014No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA8140.10194203330523874No Hit
GTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTCGCAGATGCTGGC8100.10144108965263315No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATCT8100.10144108965263315No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)