FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences798493
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG33490.4194150731440351No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG26950.33751078594302014No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG21100.26424777674945177No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT18880.23644540402984118No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC17090.2140281755757408No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC16780.21014586226804746No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT16500.2066392566998083No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG14840.18585009511667602No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT13830.17320126788838475No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC13640.17082178553850816No Hit
GTATACAGCCGTGTCCTCGGCTCTCAGTCTGTTCATTTGCAGATACAGCG13190.16518616944669523No Hit
GATTAAGACTGATGGGAGTGTAACAAGTTACGCGGACTCCGTGAAGGGCC13040.16330763074942423No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT11610.1453988951687742No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCAAGTGTTCCTG11380.142518469166292No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG11200.14026422272956682No Hit
GAAGTATACAGCCGTGTCCTCGGCTCTCAGTCTGTTCATTTGCAGATACA10550.13212388837472588No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC10370.1298696419380007No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC10000.12523591315139895No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC9930.12435926175933916No Hit
GCTGTATACTTCTGCGCACGAGATCTCAGGGGATCCAACTACTGGGGCCA9880.12373308219358214No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG9720.1217293075831598No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAAAGAAGTT9700.12147883575685699No Hit
CCCTGAGGAGACGGCGACCAGGGTTCCCTGGCCCCAGTAGTTGGATCCCC9350.11709557879655802No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9340.11697034288340662No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT9270.11609369149134684No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCA9260.11596845557819543No Hit
GTCTTAATCTGTGAGACCCATTCCGGCCCCTTCCCTGGAGGTTGGCGGAT9250.11584321966504403No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG9230.11559274783874124No Hit
GGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTG9070.11358897322831885No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA8950.11208614227050206No Hit
GTATACTTCTGCGCACGAGATCTCAGGGGATCCAACTACTGGGGCCAGGG8760.10970665992062548No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT8730.10933095218117128No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT8460.10594958252608351No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCG8320.10419627974196392No Hit
CTCCAGAGACAACGCCAAGAACACGCTGTATCTGCAAATGAACAGACTGA8230.10306915652360134No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC8000.10018873052111915No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGTG15800.046.925714
TAGGTGG16250.045.626235
GTCATAG16400.045.2684481
GAATACG2400.044.9894529
TAGGCCG803.89512E-544.9894525
GTGCTAG2900.042.2525671
CCGTTGA6350.040.809339
GTGGTCA17650.040.37588
CCCGTTG6450.040.176638
CCCCGTT6450.039.0606087
TATTGAG7550.038.1367535
AGGTGGT19450.038.1195956
GGCTAGC951.064683E-437.935741
CAGAGCG14650.037.342789
CTAGAGA3350.036.5287484
TCAACGC12500.036.2794954
ATCAACG12800.035.991563
ACAGAGC16650.035.883488
ATCTCAC6850.034.7236631
TCATAGG20800.034.607272
Produced by FastQC (version 0.11.5)