FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1275918
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGCAACTGACCAGCCTGAGTGTCGAGGACACGGCTTTTTATTACTGT20980.16443062955456383No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT20040.15706338495107053No Hit
CTGTACGACAGTGAGGGATGTCAACACTTGGAGCTTGGACTACTGGGGCC19730.15463376173076954No Hit
ACTTTGGAGAGTGGGGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGAC18450.14460176907920416No Hit
GTCGTACAGTAATAAAAAGCCGTGTCCTCGACACTCAGGCTGGTCAGTTG15850.12422428400571198No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15570.12202978561318203No Hit
TTTTATTACTGTACGACAGTGAGGGATGTCAACACTTGGAGCTTGGACTA15490.1214027860724592No Hit
GATCTATAGTGCATCTACTTTGGAGAGTGGGGTCCCGTCAAGGTTCAGTG13140.10298467456372588No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12790.10024155157306346No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26250.072.842571
ATCAACG30800.060.7743233
TCAACGC31100.060.188084
CAACGCA32600.057.418695
AACGCAG34150.054.812576
TATCAAC34350.054.7373352
ACGCAGA36900.050.5325137
CGCAGAG40300.046.2692228
GCAGAGT44200.042.5124139
GGTATCA21400.033.7174641
CCTATAC4100.029.8512543
GTACATG32050.027.4663221
TCGATTA1607.054721E-527.0582661
TACATGG31600.026.6728042
CAGAGTA46600.025.6460049
GTGTAGA4800.022.5485551
GGGAGTC9800.022.0390427
GTATTAG6250.020.7807481
CTAGTAC2908.76702E-619.8604683
CTAGCCG9800.019.8351385
Produced by FastQC (version 0.11.5)