FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831279
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCTTCAGAGTTCAGGCTGCTCAGCTCCATGTAGG29410.353792168453672No Hit
GTGTATTACTGTGCGAGAGAAGTGACTCTCGGGGCTGAACCTATAACCCC16780.20185761940335317No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15520.18670025346484154No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13980.16817458398443844No Hit
GTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGAAG13640.16408450111214165No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13550.16300183211653368No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC13490.16228005278612836No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12820.15422018359660233No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12170.14640090751721144No Hit
GTGTTACCACTGTTAGGGTTCATCCTTCCCATCCACTCAAGCCCTTGTCC12150.14616031440707633No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11320.13617570033646947No Hit
GTCTGGGGCTGAAGTGAGGGAGCCTGGGGCCTCAGTGAGGGTCTCGTGCA11200.13473214167565883No Hit
GTGGTAACACAGACTATGCACAGAAGTTCCAGGGCAGAGTCACCATGACC11000.1323262105743078No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10870.1307623553584296No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACGCATG10620.1277549414817408No Hit
CCGTAGAAGTAGCGGTTTCTCTTAGGGGTTATAGGTTCAGCCCCGAGAGT10410.12522871382532216No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10360.12462723104998442No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10170.12234159650370094No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACAGTCTCCTCAGCCTCC10130.12186041028343071No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9710.11680795497059351No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9680.11644706530539084No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCAC9470.11392083764897225No Hit
CCCTAACAGTGGTAACACAGACTATGCACAGAAGTTCCAGGGCAGAGTCA9290.1117554996577563No Hit
GTACTTATGGAGGTGTCCCTGGTCATGGTGACTCTGCCCTGGAACTTCTG9190.11055253410708077No Hit
CTCTTAGGGGTTATAGGTTCAGCCCCGAGAGTCACTTCTCTCGCACAGTA9040.1087480857810675No Hit
AACACAGACTATGCACAGAAGTTCCAGGGCAGAGTCACCATGACCAGGGA8910.1071842305651893No Hit
CTATTATGCAACCTGGTTCCAGCAGAAGCCAGGACAGGCCCCTCTACTTG8830.10622185812464889No Hit
CTGTTAGGGTTCATCCTTCCCATCCACTCAAGCCCTTGTCCAGCGGCCTG8810.10598126501451378No Hit
CAGTAATAGTCAGCCTCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGGA8740.10513918912904091No Hit
GCCTACATGGAGCTGAGCAGCCTGAACTCTGAAGACACGGCCGTGTATTA8620.10369563046823027No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG8540.10273325802768986No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8430.10140999592194679No Hit
CAGTAATACACGGCCGTGTCTTCAGAGTTCAGGCTGCTCAGCTCCATGTA8340.1003273269263388No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA8320.10008673381620371No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15500.061.362631
ATCAACG18300.050.752873
TCAACGC18600.049.931274
TATCAAC18900.049.5226142
CAACGCA19000.048.8800855
CCGCGAT450.00990900347.995959
AACGCAG19500.047.626756
GGTATCA7400.045.764411
CGCAGAG21900.042.4073838
TATTACG907.7966666E-539.9966247
ATTACGG1001.4497644E-435.9969648
TATATCG800.00210764635.9969645
GTACACG7700.035.529475
CAGCGCT4100.035.118999
ACGCAGA27900.033.2875147
GCAGCGC4800.032.9972158
CAGAGTA24950.032.606479
ACACGGG8700.031.4456217
ACGGGGT8700.031.4456219
TATGTAC9350.030.8013942
Produced by FastQC (version 0.11.5)