FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457542
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA12280.26839066140376183No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG11640.2544028744902107No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG11270.246316185180814No Hit
GTCTTTATATATCACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACC9720.2124395137495574No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8890.19429910259604583No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC8730.19080215586765806No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACATAAGTACCACTG8260.18052987485301897No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG7540.16479361457527394No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT7410.1619523453584589No Hit
GTGTATTACTGTGCGAGAGATAGTGGGGGTGATAGTAGTGGTTATGACTA7410.1619523453584589No Hit
ATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCT7300.1595481944826923No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT7140.15605124775430454No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC6920.15124294600277133No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACATA6710.14665320342176238No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG6160.13463244904292937No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT5470.11955186627675711No Hit
CATAAGTACCACTGCTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCT5290.11561780120732086No Hit
TATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTC5150.11255797281998156No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA5060.11059094028526342No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC4600.10053721844114857No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5100.059.3510551
GTATCAA10350.057.0982551
TCAACGC11750.049.632844
ATCAACG11850.049.2193763
CAACGCA12250.047.6070065
TATCAAC12500.047.2360152
AACGCAG13000.044.8604476
GAATACG1054.006297E-641.1418579
CCCCGTT1755.638867E-1141.1418577
CGCAGAG14450.040.358888
ACGCAGA14550.040.08157
CCCGTTG1807.8216544E-1139.999038
CCGTTGA1807.8216544E-1139.999039
CATCGTA800.00210603735.9991269
GCAGAGT16450.035.8897069
CAGAGCG5050.032.7912869
CCTAGGT6950.032.117842
CTAGGTC6750.032.002723
TAATACC1801.370845E-731.9992264
GTACATG11750.031.8944281
Produced by FastQC (version 0.11.5)