FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457542
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG13840.30248589200554266No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA11840.25877405790069546No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG10410.22752009651572971No Hit
GTCTTTATATATCACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACC10160.22205611725262378No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC8490.1855567357750764No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACATAAGTACCACTG7990.17462877724886458No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG7960.17397309973729186No Hit
ATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCT7530.16457505540474973No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT7220.1577997211184984No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT7210.15758116194797417No Hit
GTGTATTACTGTGCGAGAGATAGTGGGGGTGATAGTAGTGGTTATGACTA7050.1540842152195864No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACATA6830.14927591346805322No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC6240.1363809224071233No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT5710.12479728636933876No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC5690.1243601680282903No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA5590.12217457632304794No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG5460.11933330710623287No Hit
CATAAGTACCACTGCTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCT5010.10949814443264225No Hit
TATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTC4970.10862390775054531No Hit
GTGCTGGTGATATATAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCG4840.10578263853373024No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT4690.1025042509758667No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACG4670.10206713263481823No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT4590.10031865927062433No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7800.048.948171
TCAACGC7750.046.4703524
ATCAACG7800.046.1724663
CAACGCA8150.045.073395
AACGCAG8150.044.170276
TATCAAC8650.041.6398472
ACGCAGA8550.041.261757
GAATACG1755.638867E-1141.141459
ACCGTGC700.001092794641.141458
CGCAGAG9250.038.139248
GTGCTAG2300.037.5844841
GTCATAG5950.037.5318451
CCCGTTG2800.035.9987688
CCGTTGA2800.035.9987689
TATTGAG4750.034.8772245
CCCCGTT2950.034.1683247
TAGGTGG6350.034.0294725
ATAGGTG6400.033.7636154
ACCCCGT3000.033.598856
GCAGAGT10950.032.875599
Produced by FastQC (version 0.11.5)