FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802500
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA30360.37831775700934583No Hit
GTATATGAGCAGCAGTAAAAATCAGCCTCATCCTCAGCCTGGAGCCCAGA21680.2701557632398754No Hit
GTGTATTACTGTGCGAGCGGGTTCAACGGAGTTCATGCTTTTGATATCTG21300.26542056074766357No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG16880.21034267912772583No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14700.18317757009345795No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCAC14290.17806853582554516No Hit
GTACCTATGCTGTCCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAG13180.16423676012461058No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12840.16No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC12530.15613707165109034No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12320.1535202492211838No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11720.14604361370716512No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11430.14242990654205606No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11110.13844236760124612No Hit
AATAAATACTACGCAGACTCCGTGACGGGCCGATTCACCATCTCCAGAGA10900.13582554517133957No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10870.13545171339563863No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT10410.1297196261682243No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10320.1285981308411215No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10180.12685358255451715No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA9710.1209968847352025No Hit
GCTCATATACAGGCTCCACTTTCGTGCTATTCGGCGGAGGGACCAAGCTG9630.12No Hit
GGTTATAACTATGTCTCCTGGTACCAACATCACCCAGGCAAAGCCCCCAA9620.11987538940809968No Hit
GTATTTATTACTTCCATCGTATGATATAACTGCCACCCACTCCAGCCCCT9540.11887850467289719No Hit
GCATAGGTACTGAAGGTGAATCCGGAGGCTACACAGGAGAGTCTCAGGGG9440.11763239875389407No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9370.11676012461059189No Hit
GTATTACTGTGCGAGCGGGTTCAACGGAGTTCATGCTTTTGATATCTGGG9070.11302180685358255No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9070.11302180685358255No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA8930.11127725856697819No Hit
CTCATATACAGGCTCCACTTTCGTGCTATTCGGCGGAGGGACCAAGCTGA8910.11102803738317756No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8900.11090342679127725No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8830.11003115264797507No Hit
CAGTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATA8740.10890965732087228No Hit
ATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACAGCG8470.10554517133956386No Hit
CTCCTGCACTGGAGCCAGCAGTTATGTTGGTGGTTATAACTATGTCTCCT8450.10529595015576325No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC8400.10467289719626167No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTTTTACTGCTGCTCATAT8140.10143302180685358No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12800.069.799741
GGTATCA5150.064.3565441
ATCAACG15000.059.041613
TCAACGC15050.058.841794
TATCAAC15450.057.7879832
CAACGCA15950.055.5215645
AACGCAG16650.053.1873256
TCCGTAC450.00990450848.0013123
CGCAGAG19000.047.3638088
GCAGAGT21600.041.995919
CAGCCGT16050.036.3329129
GAATACG1854.476533E-935.0236139
CTAGACT1459.1817674E-734.7574044
GTAATAC14650.032.9518131
TATTGAG9200.032.8684125
TAGACTC1102.537226E-432.7261285
GTGCTAG2301.0713848E-931.3266741
AATACAC15450.031.2241523
CAGAGTA20500.030.9043069
TATCTAG1902.1917731E-730.3374081
Produced by FastQC (version 0.11.5)