FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802500
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA29390.36623052959501556No Hit
GTGTATTACTGTGCGAGCGGGTTCAACGGAGTTCATGCTTTTGATATCTG21940.2733956386292835No Hit
GTATATGAGCAGCAGTAAAAATCAGCCTCATCCTCAGCCTGGAGCCCAGA20970.26130841121495324No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16380.20411214953271026No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG16330.20348909657320874No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14420.17968847352024922No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCAC14000.17445482866043613No Hit
GTACCTATGCTGTCCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAG12870.16037383177570091No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC12670.1578816199376947No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12500.1557632398753894No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11650.14517133956386294No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10890.13570093457943924No Hit
AATAAATACTACGCAGACTCCGTGACGGGCCGATTCACCATCTCCAGAGA10800.13457943925233645No Hit
GTATTTATTACTTCCATCGTATGATATAACTGCCACCCACTCCAGCCCCT10170.12672897196261682No Hit
GGTTATAACTATGTCTCCTGGTACCAACATCACCCAGGCAAAGCCCCCAA10120.12610591900311527No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10050.1252336448598131No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT9950.12398753894080997No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT9850.12274143302180686No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9780.12186915887850468No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA9740.12137071651090343No Hit
GCATAGGTACTGAAGGTGAATCCGGAGGCTACACAGGAGAGTCTCAGGGG9690.12074766355140186No Hit
GCTCATATACAGGCTCCACTTTCGTGCTATTCGGCGGAGGGACCAAGCTG9500.11838006230529595No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9330.11626168224299065No Hit
ATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACAGCG9250.11526479750778816No Hit
GTATTACTGTGCGAGCGGGTTCAACGGAGTTCATGCTTTTGATATCTGGG9060.11289719626168225No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8980.11190031152647974No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA8940.1114018691588785No Hit
CTCCTGCACTGGAGCCAGCAGTTATGTTGGTGGTTATAACTATGTCTCCT8870.11052959501557631No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8780.10940809968847352No Hit
GTGTCCGGGTCTCCTGGACAATCAGTCACCATCTCCTGCACTGGAGCCAG8760.10915887850467289No Hit
CTCATATACAGGCTCCACTTTCGTGCTATTCGGCGGAGGGACCAAGCTGA8700.10841121495327104No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8620.10741433021806852No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC8350.10404984423676013No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8250.102803738317757No Hit
CAGTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATA8240.1026791277258567No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG9300.058.083283
GTATCAA9700.057.1945081
TCAACGC9550.056.5557174
CAACGCA10200.052.948385
GGTATCA3750.049.9548231
AACGCAG10850.049.7639436
TATCAAC11000.048.4610822
CGCAGAG12750.042.3481378
CAGCGCT4600.039.1259969
GCAGCGC5500.035.3414468
GCAGAGT15400.035.060969
GGCAGCG5900.034.1656157
CAGCCGT16200.031.9963689
GTACATG17750.030.8496761
TCTACAC4250.030.5039713
TACACCG950.004886215630.3199125
GTAATAC13300.029.7952041
TATTGAG9050.029.4404685
TACATGG18450.029.2832262
CAGTGCG1000.006286077728.7967349
Produced by FastQC (version 0.11.5)