FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417597
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTTCAGGGACA12240.29310555391920917No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG11270.26987741770175555No Hit
CTATTATACTGGGAGCACCTACCAGAATCCGTCCCTCAAGAGTCGAGTCA9900.23707066861112508No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG9090.21767397754294213No Hit
GTCTCTGGTGGCTCCATCAGCAATTATGATTACTACTGGGGCTGGATCCG8980.21503985900281852No Hit
GCTTGATACCACTGCTTGATACCACTGCTTGATACCACTGCTTGATACCA8820.2112084138535478No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG8760.2097716219225713No Hit
GTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAG7880.18869867360158238No Hit
ATCTATTATACTGGGAGCACCTACCAGAATCCGTCCCTCAAGAGTCGAGT7630.1827120405558469No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG7240.17337289300449957No Hit
GTATAATAGATAGTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCG6820.16331534948766394No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT6700.1604417656257109No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTGGGTGTTCGG6360.15229994468351066No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT6150.14727117292509284No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6000.14367919309765156No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG5880.14080560923569854No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC5640.13505844151179247No Hit
GTGTATTACTGTGCGAGAGACTCAAATCCCTATGGTTCAGGGAGTTATAA5600.1341005802244748No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC5470.13098753104069233No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT5170.12380357138580976No Hit
ATAATAGATAGTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGA5120.12260624477666267No Hit
CCCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCAAACCGT4900.11733800769641543No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCATTCAGG4310.1032095537084797No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAACGTCT4220.10105436581201493No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTCG100.0072410177143.977259
GTACCGT300.001988161672.005876
TCAACGC10950.050.6342624
ATCAACG11050.050.1760333
CAACGCA11100.049.950025
TATCAAC11350.048.8497962
AACGCAG11600.047.7976
ACGCAGA13150.041.605597
CGCAGAG13600.040.2289358
CAGAGCG4950.036.3578959
ACTTACC800.00210720935.9943128
ACCCTAT1201.0025371E-535.9943128
GCAGAGT15800.034.6274389
GTAAGGC850.002833590133.8851173
GCCCGTT900.003753705231.9949427
GCACACA5550.031.1376742
ACCCCGT2351.3533281E-930.6407956
CCGTTGA1902.2042695E-730.3109999
GTCATAG4300.030.1456031
CCCGTTG2151.9337676E-830.1347758
Produced by FastQC (version 0.11.5)