FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455259
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCTGTGTCCTCGATCTTCAGGCTATTCATTTGCAGATACA12460.2736903608715039No Hit
CCCTTAGACTCTCCTGTGCAGGCTCTGGATTCACTTTCACTAACGCCTGG10180.22360897862535395No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8980.19725035639053812No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8760.19241794231415524Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA7950.1746258723056546No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7890.1733079411939138No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC7800.1713310445263026No Hit
GTGTATTACTGTGCCACAGACATACCCATATCTGATAATAGTGGTAATTA7310.16056794044708617No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7060.15507656081483287No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT6930.15222104340606116No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA6490.14255621525329537No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG6410.14079897377097433No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT6070.13333069747110984Clontech Universal Primer Mix Long (96% over 26bp)
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC5980.13135380080349868No Hit
TCCATAGACTGGGTCCCGCTGATGGTCAGAGTGGCTGTGTTCCCAGAGTT5760.12652138672711577No Hit
GGGTATGTCTGTGGCACAGTAATACACGGCTGTGTCCTCGATCTTCAGGC5620.12344621413305393No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5400.11861380005667103Clontech Universal Primer Mix Long (96% over 26bp)
GTGTGAGGTGCACCTGGTGGAGTCTGGGGGAGGCTTGGTAAAGCCGGGGG5270.11575828264789932No Hit
CTCCTGTGCAGGCTCTGGATTCACTTTCACTAACGCCTGGATGAGCTGGG5210.11444035153615854No Hit
GACATACCCATATCTGATAATAGTGGTAATTACTACGGGGGCTGGGGCCA5200.1142206963508684No Hit
ATACACGGCTGTGTCCTCGATCTTCAGGCTATTCATTTGCAGATACAGCG5070.1113651789420967No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC5070.1113651789420967No Hit
GATATGGGTATGTCTGTGGCACAGTAATACACGGCTGTGTCCTCGATCTT4840.10631310968042366No Hit
GCTTGATACCACTGCTTGATACCACTGCTTGATACCACTGCTTGATACCA4670.10257897153049143No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG4620.10148069560404077No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)