FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3590711
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA94270.2625385334547949No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT92220.2568293577511529No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC74400.20720130358583577No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT73740.20536322750563885No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC65180.18152393773823625No Hit
AATCCATACTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA64560.1797972602083543No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG61580.17149806820988936No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG60950.16974354104242864No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT60180.16759911894886556No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC59140.16470275664067646No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG53010.147630928804908No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC52810.14707393605333316No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA50020.13930388716886433No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT49770.13860764622939578No Hit
CTCTTATTGGTGGGGATGGTAATCCATACTATGCAGACTCTGTGAAGGGC48980.13640752486067523No Hit
GTATTATTGTGCAAAAGCGGTATATTACGATATTTTGACTGGTTTCGAAG48420.13484794515626572No Hit
ATATTACTGTCAGCAGTATGATAGCTCACCGGGGTACACTTTTGGCCAGG47010.13092114625766318No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT46810.13036415350608835No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45310.12618670786927713No Hit
GGTCTGGAGTGGGTCTCTCTTATTGGTGGGGATGGTAATCCATACTATGC45210.1259082114934897No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC45160.12576896330559603No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC42280.1177482676829185No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT41510.11560384558935544No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG40680.1132923256703199No Hit
ATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACAGCAAA40310.11226188907990646No Hit
ATACTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACA40070.11159349777801666No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT39760.11073015901307567No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG38980.10855788728193386No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG38920.10839078945646141No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT38390.10691475866478813No Hit
GTCTGAGAATTGAGGACACCGCCTTGTATTATTGTGCAAAAGCGGTATAT38380.10688690902720938No Hit
CAATAATACAAGGCGGTGTCCTCAATTCTCAGACTGTTCATTTGCAGATA38240.106497014101107No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC38130.10619066808774083No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT38040.10594002134953218No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA37720.10504883294701244No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC36580.10187397426303592No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34600.065.1673361
TCAACGC36750.059.3673174
ATCAACG37800.057.7182243
CAACGCA38900.056.4539345
AACGCAG42250.051.976986
TATCAAC44300.049.411262
GGTATCA18700.043.1457631
GTACATG72350.043.1132851
TACATGG75900.040.697862
CGCAGAG54150.040.4204338
ACATGGG75700.039.6645283
GCAGAGT64100.034.2584429
CATGGGA46100.033.425274
ACGCAGA70600.030.9003757
GTACACG34700.029.4647545
TACACGG36600.028.1314986
GTATGTA40900.027.8288671
CAGCGCT25300.027.0351589
TATGTAC40400.025.3083042
ACACGGG40550.025.2129277
Produced by FastQC (version 0.11.5)