FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3590711
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT91280.2542114918187512No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA84600.23560793391615198No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT67510.188012903294083No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC61470.17119172219652318No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC59240.16498125301646385No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT56440.15718335449441628No Hit
AATCCATACTATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA56420.1571276552192588No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG55780.15534527841421936No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG49310.1373265629007737No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG48260.13440235095500586No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC47830.13320481653911997No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT45980.12805263358705282No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA45140.12571326403043856No Hit
CTCTTATTGGTGGGGATGGTAATCCATACTATGCAGACTCTGTGAAGGGC44320.12342959374898174No Hit
GTATTATTGTGCAAAAGCGGTATATTACGATATTTTGACTGGTTTCGAAG43600.12142441984331236No Hit
ATATTACTGTCAGCAGTATGATAGCTCACCGGGGTACACTTTTGGCCAGG43590.12139657020573363No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT42650.11877870427333193No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT41730.11621653761608773No Hit
GGTCTGGAGTGGGTCTCTCTTATTGGTGGGGATGGTAATCCATACTATGC41190.1147126571868357No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT39220.10922627858382367No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38660.10766669887941414No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC38540.10733250322846925No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA37690.10496528403427623No Hit
ATGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACAGCAAA36360.10126128223630361No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG36310.10112203404840991No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC36030.10034224419620515No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31300.061.420841
TCAACGC34900.053.8480454
ATCAACG37400.050.44113
CAACGCA40800.046.0611955
TATCAAC41250.045.907822
GGTATCA18650.044.0123831
GTACATG69650.043.2119641
AACGCAG43800.042.7419246
ACATGGG68850.041.41393
TACATGG74100.039.4514272
CGCAGAG50450.036.3943448
CATGGGA40950.032.8808174
GCAGAGT58950.031.2648669
TACACCC59750.026.993865
ACGCAGA68750.026.8115657
CATGGGG48550.025.9539644
GTACACG32400.025.334645
TACACGG33300.025.0823756
GTATGTA36800.025.044421
CAGCGCT25600.025.0294049
Produced by FastQC (version 0.11.5)