FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1588288
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT49080.3090119676028529No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40340.25398416408107344No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31880.200719265019946No Hit
ATAATAAACAGCCGTGTCCTCACTTGTCAGGCTGTTCATTTGCAGATACA31580.1988304388121046No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27250.17156838054559373No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTGGCAA24880.1566466535036467No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG24220.15249123584639562No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23510.14802101382117097No Hit
GTTTATTATTGTGCGAGAAAATCAAACGCTGACTACCGGCAAGTCGTCTT22820.14367671354313577No Hit
TTTAAGAGGTGTCCAGTGTCAGATGCAACTGGTGGAGTCTGGGGGAGGCG21690.13656213482693316No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21380.13461034774549702No Hit
CATCTATTCTGCATCCACTTTGCAAACTGGGGTCCCATCCAGGTTCAGCG21240.13372889551517106No Hit
GTTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCCGGAGTGGGTGGC21140.13309928677922392No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20960.1319659910545191No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20850.13127342144497722No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT20730.1305178909618407No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC19750.12434772534955878No Hit
CCCCTAGGCTCCTCATCTATTCTGCATCCACTTTGCAAACTGGGGTCCCA19120.12038119031309183No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC19070.12006638594511826No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18890.11893309022041343No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18650.11742202925414032No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA18440.11609985090865133No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18170.11439990732159407No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18140.11421102470080993No Hit
AATGAGTACTATGCAGACTCCGTGAAGGGCCGATTCATCATCTCCAGAGA18110.1140221420800258No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17910.11276292460813153No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA17390.10948895918120644No Hit
GGTATCAGCAGCGACCAGGGAAAGCCCCTAGGCTCCTCATCTATTCTGCA17240.10854454607728574No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17150.10797789821493332No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17090.10760013297336504No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC17090.10760013297336504No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC16580.10438912842003466No Hit
CCTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA16290.10256326308578796No Hit
GTACTCATTACCTCCATCAGAGGACATAACTGCCACCCACTCCGGCCCCT16280.10250030221219325No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16230.10218549784421968No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT15890.10004482814199944No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)