FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1906233
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTCCATTTGCAGATAGA61410.3221536926493246No Hit
GTGTATTACTGTGCGAAGTCTAAGGGTTTGGAGTGGTTCACAGTCGTCGG55660.2919894892177399No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT52450.27514999478028135No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAACTGGTGGAGTCGGGGGGAGGCT38080.19976571594343398No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37740.19798209347965334No Hit
GTATAGTCACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGTTGGAGA36920.19368041577288822No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT31270.16404080718359193No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC30340.15916207515030953No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT29840.1565391009388674No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29820.1564341819704097No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC29560.1550702353804598No Hit
GTTCACAGTCGTCGGCTACTACTTTGACTACTGGGGCCAGGGAACCCTGG29400.1542308836327983No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA29030.15228988271633112No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28520.14961444902066012No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA26800.1405914177332991No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC25970.13623728054230516No Hit
GTCTAAGGGTTTGGAGTGGTTCACAGTCGTCGGCTACTACTTTGACTACT25810.13539792879464368No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG25470.13361430633086302No Hit
ACTGTGAACCACTCCAAACCCTTAGACTTCGCACAGTAATACACAGCCGT25450.13350938736240534No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGCGTCACCCTCTCCT25290.13267003561474386No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC23950.12564046472807888No Hit
CCCTTAGACTTCGCACAGTAATACACAGCCGTGTCCTCAGCTCTCAGGCT23520.12338470690623864No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22370.1173518662199217No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT22030.11556824375614104No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG21620.11341740490275846No Hit
AATACATACTACTCAGACTCCGTGAGGGGCCGGTTCACCGTCTCCAGAGA21400.11226329624972393No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT21210.11126656604937592No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20770.10895834874330682No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG20110.10549602278420318No Hit
GTATTACTGTGCGAAGTCTAAGGGTTTGGAGTGGTTCACAGTCGTCGGCT20070.10528618484728781No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19980.10481404948922822No Hit
GCTGTGTATTACTGTGCGAAGTCTAAGGGTTTGGAGTGGTTCACAGTCGT19830.10402715722579559No Hit
ATACTACTCAGACTCCGTGAGGGGCCGGTTCACCGTCTCCAGAGACAATT19450.10203369682509957No Hit
TCTATGATGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGC19230.10087958817206501No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG19070.10004023642440352No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)