FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577474
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCCTCTCAGGCTGC17440.30200493875048917No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC17290.29940741920848385No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA17010.2945587160634072No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCCTCTCAGGCTG16910.29282703636873697No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG16460.2850344777427209No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT15740.27256638394109517No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA14210.2460716846126406No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACT14030.24295466116223416No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT13990.24226198928436604No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC13720.23758645410875642No Hit
TCCTATGACAGCAGCCTGAGAGGAGTCTTCGGAACTGGGACCAAGGTCAC13080.2265037040628669No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT12800.22165500091779022No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAGCAATCGGCCCT12230.21178442665816988No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11260.1949871336198686No Hit
GGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACT10740.18598239920758336No Hit
GTGTACATCATAACCTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGA9780.1693582741387491No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC9230.15983403581806283No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG9090.15740968424552448No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT8550.1480586138943052No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC8080.1399197193293551No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8080.1399197193293551No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA8010.13870754354308593No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC7990.13836120760415188No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCC7980.13818803963468487No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC7970.13801487166521784No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGAGAGGAGTCTTCGGAAC7570.13108815288653689No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG7420.12849063334453154No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC7330.12693212161932832No Hit
CCTCTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCCTCATCCTC7100.12294925832158678No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT6960.12052490674904844No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA6880.11913956299331227No Hit
GGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAAC6760.11706154735970796No Hit
GTTCCTGGAAGCTGCTGGTACCAGTGTACATCATAACCTGCCCCGATGTT6730.11654204345130689No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT6650.11515669969557071No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCT6340.10978849264209296No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC6240.10805681294742274No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT6220.10771047700848868No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG6200.10736414106955465No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15600.061.412281
TCAACGC15600.059.529194
ATCAACG15750.058.9622463
CAACGCA16150.057.501885
TATCAAC16750.055.4421122
AACGCAG16850.055.113086
CGCAGAG19250.048.6158038
GCAGAGT21850.042.8308569
GGTATCA8050.041.16141
GAATACG1755.638867E-1141.136459
ACGCAGA23100.040.5131727
GTACTAG800.002101589236.0162241
GTACATG16200.032.014421
TACATGG16050.031.3969782
ACATGGG17250.029.6301673
TGCTAGA3002.0008883E-1128.7955152
TACGAGT1907.5524786E-626.5221846
GTGCTAG3306.730261E-1126.1936171
TATTGGG4950.026.177742
ATATACG2207.95304E-726.177743
Produced by FastQC (version 0.11.5)