FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577474
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16690.2890173410404624No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC15840.2742980636357654No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCCTCTCAGGCTG15610.27031520033802386No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCCTCTCAGGCTGC15600.27014203236855683No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT15110.2616568018646727No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCC15010.25992512217000246No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG14530.25161305963558533No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT13490.23360359081101484No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACT13470.23325725487208082No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA13060.22615736812393286No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC12730.22044282513152108No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT12260.21230393056657096No Hit
TCCTATGACAGCAGCCTGAGAGGAGTCTTCGGAACTGGGACCAAGGTCAC11840.20503087584895596No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAGCAATCGGCCCT11330.19619930940613775No Hit
GGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACT10670.18477022342131422No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC10550.18269220778770992No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC8960.15515850064245318No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC8690.15048296546684353No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA8520.14753910998590414No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG8310.14390258262709663No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGAGAGGAGTCTTCGGAAC7940.13749536775681676No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCC7870.13628319197054758No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC7670.1328198325812071No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGT7640.13230032867280606No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC7600.13160765679493797No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC7500.12987597710026771No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT7470.12935647319186663No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA7430.12866380131399854No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG7310.12658578568039427No Hit
GTGTACATCATAACCTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGA7290.1262394497414602No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT6960.12052490674904844No Hit
TCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTGCTG6530.11307868406196643No Hit
CCTCTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCCTCATCCTC6240.10805681294742274No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG6240.10805681294742274No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG6070.10511295746648333No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCC6020.10424711761914823No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCA5900.10216910198554395No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCT5890.10199593401607691No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT5880.10182276604660989No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGT5830.10095692619927477No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT5820.10078375822980776No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)