FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735934
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATACACAGCCGTGTCGTCGACTCGCAAACTGTTCACTTCCAGCCACA26220.3562819491965312No Hit
CAATAATACACAGCCGTGTCGTCGACTCGCAAACTGTTCACTTCCAGCCA17690.24037481621993276No Hit
GTGTATTATTGTGTAGAGAATACACCGTTGACTCTGTGGGGCCAGGGAAC17530.23820070821568237No Hit
CTCCTGTGTCGCGTCTGGATTCAGCATTGAAACCTCTGTCATGCACTGGG17250.23439601920824424No Hit
GTATGAGGGCACTGAGAAGTTCTATGCGGACTCCATTAAGGGCCGATCCA16400.22284607043566407No Hit
GTATTATTGTGTAGAGAATACACCGTTGACTCTGTGGGGCCAGGGAACCC16110.21890549967796025No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16040.2179543274261007No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15750.21401375666839692No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT15470.21020906766095873No Hit
ATACACAGCCGTGTCGTCGACTCGCAAACTGTTCACTTCCAGCCACATCG15240.20708378740484878No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14750.200425581641832No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14460.1964850108841282No Hit
CTCCAGGCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGACAGAGGTTT13400.18208154535596943No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG13040.17718980234640605No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA13020.17691803884587476No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13010.17678215709560913No Hit
GCTGTGTATTATTGTGTAGAGAATACACCGTTGACTCTGTGGGGCCAGGG12690.17243394108710836No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC12570.17080336008392058No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12490.1697163060817954No Hit
GGCCTGGAGTGGGTGGCTGCCATATGGTATGAGGGCACTGAGAAGTTCTA12340.16767807982781066No Hit
ATTAAGGGCCGATCCACCGTCTCCAGAGACAATTCCAAGAACACGATGTG12300.16713455282674805No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11160.1516440332964641No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA11070.15042109754407326No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC11040.1500134522932763No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10880.14783934428902593No Hit
GTGTATTCTCTACACAATAATACACAGCCGTGTCGTCGACTCGCAAACTG10740.14593699978530683No Hit
GTCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGCCTGGAGTGGGTGGC10600.14403465528158776No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10360.14077349327521219No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA10330.14036584802441524No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCAGCACTAGAAGTCGGCGGTGTT9950.1352023415143206No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCA9540.129631189753429No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG9470.12868001750156943No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9450.12840825400103814No Hit
CTATACAAGCTTCAATTTCGGCCAGGGGACCAGGGTGGAGATCGAACGAA9440.12827237225077248No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9430.12813649050050685No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC9280.12609826424652212No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGT9120.12392415624227172No Hit
CCATTAAGGGCCGATCCACCGTCTCCAGAGACAATTCCAAGAACACGATG9090.12351651099147477No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC8800.11957594023377097No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG8760.11903241323270837No Hit
CCCTCATACCATATGGCAGCCACCCACTCCAGGCCCTTGCCTGGAGCCTG8750.11889653148244271No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8620.11713006872898929No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8600.11685830522845798No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8590.11672242347819234No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG8360.11359714322208242No Hit
CCATATGGCAGCCACCCACTCCAGGCCCTTGCCTGGAGCCTGGCGGACCC8300.11278185272048853No Hit
CCCTCAGACTCTCCTGTGTCGCGTCTGGATTCAGCATTGAAACCTCTGTC8140.11060774471623813No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8030.10911304546331599No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT8000.10870540021251905No Hit
GCCATATGGTATGAGGGCACTGAGAAGTTCTATGCGGACTCCATTAAGGG7940.10789010971092516No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC7940.10789010971092516No Hit
GAATACACCGTTGACTCTGTGGGGCCAGGGAACCCTGGTCTCCGTCTCCT7840.10653129220826868No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGAGACCAGGGTTCCCTGG7790.10585188345694042No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC7790.10585188345694042No Hit
CCCTCCACCCTGTCTGCCTCTGTGGGAGACAGAGTCATCATCACTTGCCG7650.10394953895322134No Hit
GAGAATACACCGTTGACTCTGTGGGGCCAGGGAACCCTGGTCTCCGTCTC7480.10163954919870531No Hit
TCTCTACACAATAATACACAGCCGTGTCGTCGACTCGCAAACTGTTCACT7450.10123190394790836No Hit
GTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTCAGACTCTCCTGTG7380.10028073169604883No Hit
ATTAGTGACTGGTTGGCCTGGTTTCAACAGAAACCAGGGAAAGCCCCCAA7380.10028073169604883No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18750.056.5695341
TCAACGC20600.051.3738144
GGTATCA9500.050.8882751
ATCAACG20850.050.7578243
CAACGCA21200.049.919845
AACGCAG22000.048.4318166
TATCAAC21950.047.925242
GAATACG2750.044.5049139
ACGCAGA24000.044.3958327
CGCAGAG24750.043.6322678
GCAGAGT26300.041.0607839
GTGCTAG3450.039.737551
ATTACGG850.002837426733.8791733
TGCTAGA4500.030.4219472
CAGTGCG950.00489145230.3129449
GCTAGAG4300.030.1367053
GTACATG21250.028.862011
TACCGCA1000.006285144528.7972957
GAGTTTA4350.028.1985131
TACATGG21850.027.699622
Produced by FastQC (version 0.11.5)