FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1081983
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATTACTGTGCGAGAGATCGCGGTCGGGATGGTTCGGGGAGTTATCC55520.513131906878389No Hit
GTAATAGACAGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATACA53720.4964957859781531No Hit
ATACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGACAACG29600.27357176591499127No Hit
TACATATACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGA20980.1939032313816391No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT20250.1871563601276545No Hit
GTATATGTAATTATTACTACTACTAATGGATGAGACCCACTCCAGCCCCT19650.1816109864942425No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT18040.16673090057792037No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17450.16127794983839858No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17350.16035372089949657No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17120.15822799434002197No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16030.1481538989059902No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15690.1450115205137234No Hit
GCGTAGTATATGTAATTATTACTACTACTAATGGATGAGACCCACTCCAG15560.1438100228931508No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15210.14057522160699384No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA14660.13549196244303285No Hit
TTACATATACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAG14430.13336623588355823No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14340.13253442983854646No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14130.13059354906685225No Hit
CTATTATTGTCAGCAGTGTGGTAGCTCACCATTCACTTTCGGCCCTGGGA14050.12985416591573065No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG13860.12809813093181685No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC13420.12403152360064808No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC13280.12273760308618527No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13140.12144368257172247No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13080.12088914520838127No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG12980.11996491626947929No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCTTAGCCCTGGATTCCAAGGCCTA12870.11894826443668707No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12810.11839372707334588No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA12720.11756192102833408No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT12580.11626800051387129No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC11770.10878174610876512No Hit
TAATTACATATACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCA11620.10739540270041212No Hit
TTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCC11420.10554694482260811No Hit
CTATTACTGTGCGAGAGATCGCGGTCGGGATGGTTCGGGGAGTTATCCCC11160.10314394958146292No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11110.10268183511201191No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT11030.10194245196089033No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC10950.10120306880976873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8100.074.780441
GTATCAA25050.070.526471
ATCAACG25100.069.172763
TCAACGC25250.068.761834
CAACGCA26450.065.6422045
TATCAAC26900.064.54412
AACGCAG27850.062.2819066
CGCAGAG32150.053.9493338
GCAGAGT37050.046.8143359
ACAGCCG22500.038.0640038
GTAATAG23600.037.8881531
CAGCCGT22650.037.8119249
TAGACAG24200.037.509995
ATAGACA24450.037.1264534
GACAGCC25600.034.0169567
AGACAGC29000.031.5192476
GTGTAGA5200.030.5079571
CCGATCG950.00490027130.3030559
GTACATG29200.029.881081
TAGCTCG1255.380354E-428.78799
Produced by FastQC (version 0.11.5)