FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1081983
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATTACTGTGCGAGAGATCGCGGTCGGGATGGTTCGGGGAGTTATCC55720.514980364756193No Hit
GTAATAGACAGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATACA48020.4438147364607392No Hit
ATACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGACAACG27870.25758260527198673No Hit
TACATATACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGA19560.18077918044923072No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT19250.17791407073863452No Hit
GTATATGTAATTATTACTACTACTAATGGATGAGACCCACTCCAGCCCCT17530.16201733298952015No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17250.15942949196059455No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT15830.1463054410281862No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14280.13197989247520525No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14200.13124050932408365No Hit
TTACATATACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAG13990.12929962855238944No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13740.12698905620513445No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG13620.12587998147845206No Hit
CTATTATTGTCAGCAGTGTGGTAGCTCACCATTCACTTTCGGCCCTGGGA13390.12375425491897747No Hit
GCGTAGTATATGTAATTATTACTACTACTAATGGATGAGACCCACTCCAG13230.12227548861673428No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT13230.12227548861673428No Hit
CTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGA13040.12051945363282049No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCTTAGCCCTGGATTCCAAGGCCTA13000.12014976205725968No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12900.11922553311835768No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA12430.1148816571055183No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12110.11192412450103191No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA11900.10998324372933771No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG11840.10942870636599651No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC11590.10711813401874151No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11410.10545452192871792No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA11380.10517725324704731No Hit
TAATTACATATACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCA10950.10120306880976873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5950.059.2525631
TCAACGC13100.057.064724
ATCAACG13450.055.574623
GTATCAA14850.053.2959561
CAACGCA15100.050.4655345
TATCAAC15500.049.615522
AACGCAG16100.047.3463756
CGCAGAG18250.041.3764538
TACATGG27150.039.4440842
GTACATG27800.039.3393171
ACAGCCG21800.037.6075638
CAGCCGT21900.036.7790769
GTAATAG23100.036.4419751
GAATACG3600.035.9581079
GCAGAGT22000.033.9967589
GTGCTAG4250.033.858611
TAGACAG24300.033.1342935
ATAGACA24950.032.5546884
GACAGCC24850.032.1235437
ACATGGG36200.029.5830653
Produced by FastQC (version 0.11.5)