FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences897081
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT24950.278124271944228No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20180.2249518159452714No Hit
CCATAATAGTCAGTGGATAGTTCCCCCACTCTCGCACAAAAATACACGGC18900.21068331622228093No Hit
CTATTATGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT17910.1996475234677805No Hit
GCATAAAGCAGGGAAAGCCCCTAGGCTCCTGATCTATGCTGCAGTCAGTT14980.16698603582062266No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG14690.16375332885213265No Hit
TATCTACTCTGGTCCCACCGCCGAAAGTGAGCGGGAGACTGTCACTCTGT13890.1548355165252636No Hit
GAGTAGATATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG13750.15327489936806152No Hit
GTACTGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT12650.1410129074186166No Hit
GCATGGACATGAGGGTCTCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG12620.14067848945635902No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12310.13722283717969727No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12020.13399013021120726No Hit
GTCTACAGAGTGACAGTCTCCCGCTCACTTTCGGCGGTGGGACCAGAGTA11540.12863944281508582No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11530.12852797016099995No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10770.12005604845047436No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10590.11804954067692883No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG10450.11648892351972676No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10360.115485669632954No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10170.11336768920532259No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10050.11203001735629224No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT9920.11058087285317603No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA9470.1055646034193122No Hit
ACTGTCTACAGAGTGACAGTCTCCCGCTCACTTTCGGCGGTGGGACCAGA9400.10478429484071115No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9080.10121716990996354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16950.072.759921
TCAACGC20150.061.129884
ATCAACG20550.059.9400063
TATCAAC22500.055.3854982
ACGCAGA24050.050.5783967
CGCAGAG25050.048.2719658
CAACGCA25850.047.6505665
AACGCAG26550.046.0895086
GCAGAGT30700.039.622499
AATACCG1102.530092E-432.7423485
TACATGG23800.030.8713552
GAATACG2809.094947E-1230.8472659
TATAGAC2351.3533281E-930.6524093
GTACATG24750.030.3056721
CCTAAGG1358.392809E-426.678952
ATAATAG16100.026.3972453
TAATAGT16750.025.8029234
TCCGACC1400.001042502525.7060558
GACTATG2851.0684744E-825.255077
CATAATA16800.024.8685912
Produced by FastQC (version 0.11.5)