FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences897081
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT22210.24758076472470156No Hit
CTATTATGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT18480.20600146475067468No Hit
CCATAATAGTCAGTGGATAGTTCCCCCACTCTCGCACAAAAATACACGGC17320.19307063687671458No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17140.19106412910316906No Hit
GAGTAGATATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG13600.15160280955677358No Hit
TATCTACTCTGGTCCCACCGCCGAAAGTGAGCGGGAGACTGTCACTCTGT13020.14513739561979352No Hit
GCATAAAGCAGGGAAAGCCCCTAGGCTCCTGATCTATGCTGCAGTCAGTT12990.14480297765753594No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG12790.14257352457581868No Hit
GTACTGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT11960.13332129428669207No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11250.12540673584659578No Hit
GTCTACAGAGTGACAGTCTCCCGCTCACTTTCGGCGGTGGGACCAGAGTA10990.12250844684036336No Hit
GCATGGACATGAGGGTCTCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG10850.12094782968316128No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10640.11860690394735816No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10630.1184954312932723No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9640.10745963853877186No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT9480.10567607607339805No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9210.10266631441307975No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT9050.10088275194770596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12950.057.2763021
TCAACGC13650.051.096564
ATCAACG13650.051.0908623
ACGCAGA16200.043.960927
CGCAGAG16600.043.3349658
TATCAAC16450.043.268662
CAACGCA18100.038.54065
AACGCAG18900.037.678686
GAATACG2201.6370905E-1135.9680259
GTACATG26350.034.4347881
GCAGAGT21000.033.912719
TACATGG26200.033.4782452
GTATTAG3550.030.427791
ACATGGG33400.026.2613773
CATGGGG17000.024.9548954
TAATAGT15600.024.889824
ATAATAG14500.024.791693
GTGCTAG3652.382876E-1023.6753231
CCTTATA3102.5966074E-823.2298181
AATAGTC15250.023.1074285
Produced by FastQC (version 0.11.5)