FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883770
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAACTCAGTTGCAGGGAAA38130.431447095963882No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT27280.30867759711237086No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTAGCCACTACTGGAGCTGGATCCG24760.28016339092750375No Hit
GTCTATTACTGTGCGAGACTCCTTGATAGTAGTGGCTATTACTCCTTCGA20200.22856625592631566No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18940.21430915283388213No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15640.17696912092512757No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA14730.16667232424725892No Hit
GTATTATTGTCAAGAGAGTTACAGTTCCCCATTCACCTTCGGCCCTGGGA14310.16191995654978106No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT13850.15671498240492437No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT13520.1529809792140489No Hit
CCTTGATAGTAGTGGCTATTACTCCTTCGACCTCTGGGGCCAGGGAATCC13190.14924697602317344No Hit
GACTAGGGAAAGCCCCCAAGCTCCTCATCTCTGCTGCATCCAGTCTGCAG13010.14721024700996865No Hit
TATCTATACCGGAGGGAGTGCCTATTACAGACCCTCCCTCAAGAGTCGAC12990.14698394378627924No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT12800.14483406316122974No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12800.14483406316122974No Hit
CTATCAAGGAGTCTCGCACAGTAATAGACGGCCGTGTCTGCGGCGGTCAC12660.14324994059540377No Hit
GCCTATTACAGACCCTCCCTCAAGAGTCGACTCACCATATCGATGGACAA12520.14166581802957784No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12490.14132636319404368No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG12200.1380449664505471No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11850.1340846600359822No Hit
GTAATAGGCACTCCCTCCGGTATAGATACGCCCAATCCACTCCAGTCCCT11830.1338583568122928No Hit
GTCTGCATCTGTGGGAGACAAAGTCACCATCACTTGCCGGGCAAGTCAGA11390.1288796858911255No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10940.12378786335811355No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG10880.12310895368704527No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTAGCCACTACTGGA10660.12061961822646163No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10570.11960125371985925No Hit
GTCTGCAGCCTGTGGACTTTGCAACGTATTATTGTCAAGAGAGTTACAGT10310.11665931181189676No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT10290.11643300858820735No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCT10190.11530149246976024No Hit
ATTACAGACCCTCCCTCAAGAGTCGACTCACCATATCGATGGACAAGTCC10000.11315161184471073No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT9890.11190694411441893No Hit
TCCCTAGTCTCTGCTGATACCAAGCTAAATGGATGCTAATGTTCTGACTT9410.10647566674587279No Hit
GTCCACAGGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATC9380.10613621191033866No Hit
CAATAATACGTTGCAAAGTCCACAGGCTGCAGACTGCTGATGGTGAGAGT9350.10579675707480453No Hit
GAGTTACAGTTCCCCATTCACCTTCGGCCCTGGGACCAAAGTGGAAATCA9310.10534415062742569No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9170.10376002806159973No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9070.10262851194315263No Hit
TTACAGACCCTCCCTCAAGAGTCGACTCACCATATCGATGGACAAGTCCA8940.10115754098917139No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG8900.10070493454179255No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30200.033.227171
TAGACGG17450.032.5938875
GTGCTAG2455.820766E-1132.412471
ATAGACG17150.032.3244484
TCAACGC32250.031.0305584
ATCAACG32600.030.6974093
GTACACG6350.030.61225
AACGCAG32700.030.6035336
CAACGCA32600.030.4765645
AGACGGC18000.030.398046
GACGGCC18350.030.2105887
AACACCG1204.2271448E-429.9980665
AGCGCTG2901.2732926E-1129.7911855
TACACGG6550.029.6774776
TATCAAC34050.029.2101352
AAGCGCT2303.731475E-828.1720964
AATAGAC19950.028.1485633
CGCAGAG35650.028.0711238
TAATAGA20950.027.5218472
GTGCTAA2105.174679E-727.5014881
Produced by FastQC (version 0.11.5)