FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883770
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCTGCGGCGGTCACAGAACTCAGTTGCAGGGAAA32020.3623114611267637No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT22620.25594894599273565No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTAGCCACTACTGGAGCTGGATCCG22160.25074397184787894No Hit
GTCTATTACTGTGCGAGACTCCTTGATAGTAGTGGCTATTACTCCTTCGA18110.20491756905077113No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16440.18602124987270444No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14160.1602226823721104No Hit
GTATTATTGTCAAGAGAGTTACAGTTCCCCATTCACCTTCGGCCCTGGGA12690.1435893954309379No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT12280.13895017934530476No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA12140.13736605677947883No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT11630.13159532457539858No Hit
GCCTATTACAGACCCTCCCTCAAGAGTCGACTCACCATATCGATGGACAA11080.12537198592393947No Hit
TATCTATACCGGAGGGAGTGCCTATTACAGACCCTCCCTCAAGAGTCGAC10990.12435362141733708No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT10780.12197743756859818No Hit
GTAATAGGCACTCCCTCCGGTATAGATACGCCCAATCCACTCCAGTCCCT10760.12175113434490874No Hit
CCTTGATAGTAGTGGCTATTACTCCTTCGACCTCTGGGGCCAGGGAATCC10490.11869604082510156No Hit
CTATCAAGGAGTCTCGCACAGTAATAGACGGCCGTGTCTGCGGCGGTCAC10460.11835658598956741No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG10350.1171119182592756No Hit
GACTAGGGAAAGCCCCCAAGCTCCTCATCTCTGCTGCATCCAGTCTGCAG10340.11699876664743089No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10090.11416997635131314No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9920.11224639894995304No Hit
GTCTGCATCTGTGGGAGACAAAGTCACCATCACTTGCCGGGCAAGTCAGA9840.11134118605519536No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9770.11054912477228238No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9630.10896500220645643No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG9390.10624936352218338No Hit
GTCCACAGGCTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATC9280.10500469579189156No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCT9070.10262851194315263No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT9070.10262851194315263No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)