FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724915
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGAGGGCCGTGTCCTCGACTCTCAGACTATTCATTTGTAGATACA21440.2957588131022258No Hit
CTCTATTACTGTGCGAGAGTGGCCCCGGGAGATGGCTACAATCTAAACCA15810.2180945352213708No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11190.15436292530848444No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10810.14912093141954574No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA10530.14525840960664355No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10130.13974052130249753No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9600.13242931929950408No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9370.12925653352462013No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8810.12153148989881572No Hit
CTATTACTGTGCGAGAGTGGCCCCGGGAGATGGCTACAATCTAAACCACT8620.11891049295434637No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8510.11739307367070623No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8380.11559975997185877No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8110.11187518536656023No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)