FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724915
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGAGGGCCGTGTCCTCGACTCTCAGACTATTCATTTGTAGATACA20940.2888614527220433No Hit
CTCTATTACTGTGCGAGAGTGGCCCCGGGAGATGGCTACAATCTAAACCA16370.2258195788471752No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13100.1807108419607816No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA10570.14581019843705814No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10140.1398784685101012No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9970.13753336598083915No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9240.1274632198257727No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9170.12649758937254713No Hit
CTATTACTGTGCGAGAGTGGCCCCGGGAGATGGCTACAATCTAAACCACT8500.11725512646310256No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8400.11587565438706607No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7560.10428808894835946No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7430.102494775249512No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT554.2327647E-665.449226
TCAACGC9500.043.966454
CAACGCA9700.043.059925
GTATCAA10400.042.244821
ATCAACG10200.040.9491423
AACGCAG10250.040.7381486
TATCAAC11250.037.767352
CGCAGAG11450.036.4686438
GGGCCGT11350.032.349799
TAGAGGG14100.030.1335225
GTAATAG13350.029.6727891
GTATAAG3301.8189894E-1228.3730721
GTACACG5600.028.2912185
ACGCAGA15100.027.6533787
TAATAGA14250.027.2895222
GCAGAGT15050.027.266889
AATAGAG14050.027.1654263
GGTATCA6200.026.7185631
TACACGG5950.026.6196786
ATAGAGG14700.026.4541284
Produced by FastQC (version 0.11.5)