FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences942350
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT17170.1822040643073168No Hit
GTATTACTGTCAGCAGTATGGTAACTCACCTGGAACTTTTGGCCAGGGGA14920.15832758529208893No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11850.12574945614686686No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11470.12171698413540616No Hit
GTCTCCAGGCACCCTGTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT10520.11163580410675439No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC10180.10802780283334218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7650.066.0142061
GTATCAA19850.064.331
TCAACGC20750.060.1138884
ATCAACG21100.059.458453
CAACGCA22000.056.6983225
TATCAAC22750.056.0968742
AACGCAG22650.054.9893846
ACGCAGA25000.049.8098077
CGCAGAG26650.046.72598
GCAGAGT32100.038.7926839
GTACATG23000.033.2490161
TACATGG27400.027.1040042
CAGAGTA32300.026.2959149
ACATGGG27400.025.7882753
GAATACG3301.9263098E-923.9931629
GTGTAGA5250.023.3609431
GGACCGT1650.002708500521.8165976
CATTAGC5300.021.7296588
GTCTAGG2404.531147E-521.0420271
TAAGACC3851.1197699E-820.6005464
Produced by FastQC (version 0.11.5)