FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences942350
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT15640.16596805857696184No Hit
GTATTACTGTCAGCAGTATGGTAACTCACCTGGAACTTTTGGCCAGGGGA15460.16405794025574363No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA10460.11099909799968165No Hit
GTCTCCAGGCACCCTGTCCTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT9680.10272191860773597No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9620.10208521250066323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG13800.058.87553
TCAACGC14250.057.0193064
GTATCAA14850.056.180081
TATCAAC16100.050.46742
CAACGCA16300.050.28935
AACGCAG17350.047.2106866
ACGCAGA17450.046.615147
GGTATCA6400.046.073871
CGCAGAG18500.043.5803078
GAATACG2750.036.647079
TACCGTC1406.9881025E-735.992667
GCAGAGT24500.032.9075789
GATACCG1602.0016505E-631.4583855
GTACATG24700.029.6998331
GTGCTAG4850.029.6578081
TAGACTG4400.027.7814315
ATACCGT2105.3932854E-727.3719446
TACATGG27850.027.109562
GTCTAGC1907.6152446E-626.4969121
CTAGTAC1658.9066096E-526.1458383
Produced by FastQC (version 0.11.5)