FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences795607
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATAATGATCTAGTGCCCTCAGGGGTCTCTGACCGATTCTCTGGCT28010.35205823980935314No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCGGTGTCT26990.3392378397877344No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCGGTGTCTGAAGCCC25910.3256632985883734No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCG21670.2723706553612525No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21630.27186789457609095No Hit
TCCTCACCCTCATCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG21460.2697311612391545No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA19820.24911796904753228No Hit
TTATAATAGATGACGAGTTTGGGAGCCTTTCCTGGGAGCTGCTGGTACCA18440.2317727219594599No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT17620.22146612586364878No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA16750.21053107878638574No Hit
CATCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC16140.2028639768126726No Hit
GTAATACACGGCCGTGTCCTCGGATCTCAGGCTGCGCAGCTCCATGTAGG16090.2022355258312207No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA15810.19871620033509005No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15780.19833912974621892No Hit
GTGCTGACTCAGCCACCCTCGGTGTCTGAAGCCCCCAGGCAGAGGGTCAC15600.19607670621299209No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14720.1850159689394387No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA14500.18225078462105035No Hit
ATTATAATGATCTAGTGCCCTCAGGGGTCTCTGACCGATTCTCTGGCTCC14120.17747455716201593No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA13750.17282401989927187No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC13640.1714414277400777No Hit
GCCTACATGGAGCTGCGCAGCCTGAGATCCGAGGACACGGCCGTGTATTA13540.1701845257771739No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC13530.17005883558088353No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13510.16980745518830276No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13310.16729365126249518No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT13030.16377432576636455No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12920.16239173360717038No Hit
GTCTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC12850.16151190223313772No Hit
GAAATAGTCAGCCTCATCCTCAGTCTGGAGCCCACTGATGGCCAGGGAGG12590.15824395712958786No Hit
GTGTATTACTGTGCGGCATGGTCGTGTGACTCCGGCGACTACCAGTGTCA12420.1561072237926514No Hit
TTTCTGTGCAGCGTGGGATGACAGCCTGGGTGGCCGGGTGTTCGGCGGAG12250.15397049045571495No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT12150.15271358849281114No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11890.14944564338926128No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA11790.1481887414263575No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT11700.14705752965974406No Hit
GTCTGGGCCTGAGGTGAAGAAGCCTGGGACCTCAGTGAAGGTCTCCTGCA11340.1425326825932904No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG10680.13423712963812534No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA10390.13059211394570433No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10350.13008935316054282No Hit
GTTCCTCACTATGGACTGGATTTGGAGGATCGTCTTCTTGGTGGGAGCAG10320.1297122825716717No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC10170.12782692962731598No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10160.12770123943102563No Hit
GACTATTTCTGTGCAGCGTGGGATGACAGCCTGGGTGGCCGGGTGTTCGG10130.12732416884215447No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9950.12506174530892764No Hit
GCACAGAAATAGTCAGCCTCATCCTCAGTCTGGAGCCCACTGATGGCCAG9910.12455898452376614No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGGCCACCCAGG9860.12393053354231424No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG9670.12154241981279704No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9660.12141672961650664No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGGCCACCCAGGC9650.12129103942021627No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG9610.12078827863505473No Hit
CCTCAGGGGTCTCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCC9340.11739464333521449No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9290.1167661923537626No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCGGTGT9280.11664050215747222No Hit
GCTCCAGACTGAGGATGAGGCTGACTATTTCTGTGCAGCGTGGGATGACA9190.1155092903908588No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG9100.11437807862424539No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT9010.11324686685763197No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8870.11148720410956667No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA8750.1099789217540821No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCCCCTCTCTTCCTCACCCTCA8730.10972754136150135No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8580.10784218841714566No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC8520.10708804723940338No Hit
GATCTAGTGCCCTCAGGGGTCTCTGACCGATTCTCTGGCTCCAAGTCTGG8460.10633390606166108No Hit
CTCCAGCATGGCCTGGTCCCCTCTCTTCCTCACCCTCATCACTCACTGTG8360.10507700409875731No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG8230.10344303154698237No Hit
AGTCTGTGCTGACTCAGCCACCCTCGGTGTCTGAAGCCCCCAGGCAGAGG8130.10218612958407856No Hit
GTTCTGGAAGCACCTCCAACATCGGAAATAACGCTGTCAACTGGTACCAG8120.1020604393877882No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG8090.10168336879891705No Hit
GTATTACTGTGCGGCATGGTCGTGTGACTCCGGCGACTACCAGTGTCAAA8060.10130629821004593No Hit
CCCTCATCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAG8030.10092922762117476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13150.077.877371
GGTATCA5050.072.832881
TCAACGC17350.059.414694
TATCAAC17250.059.341232
ATCAACG17550.058.73763
CAACGCA18250.056.484655
AACGCAG19450.054.410326
TTAACGC450.009858374548.0580334
CGCAGAG21600.047.9825338
ACGCAGA24050.043.393767
GCAGAGT25850.040.093729
CAGAGTA25100.034.9832769
TATTGAG13600.033.3932655
GTACATG21150.032.7347871
ATTGAGT9750.031.011896
TTATTGA14650.030.507834
GTAATAC9550.030.2068391
ACATGGG22450.030.1834433
GGGTTTA7050.029.6659031
CATGGGA13000.028.2803064
Produced by FastQC (version 0.11.5)