FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences795607
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCGGTGTCTGAAGCCC27250.3425057848912843No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCGGTGTCT25350.31862464759611214No Hit
CTATTATAATGATCTAGTGCCCTCAGGGGTCTCTGACCGATTCTCTGGCT24130.30329044364868585No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23140.29084711421593823No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCG22630.2844369142051289No Hit
TCCTCACCCTCATCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG19720.2478610670846285No Hit
TTATAATAGATGACGAGTTTGGGAGCCTTTCCTGGGAGCTGCTGGTACCA18320.23026443960397536No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17150.2155586866380009No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA15820.19884189053138043No Hit
GTGCTGACTCAGCCACCCTCGGTGTCTGAAGCCCCCAGGCAGAGGGTCAC15680.1970822277833151No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA15600.19607670621299209No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT15510.19494549444637868No Hit
CATCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC15150.190420647379925No Hit
GTAATACACGGCCGTGTCCTCGGATCTCAGGCTGCGCAGCTCCATGTAGG15010.1886609846318597No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14890.18715270227637515No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14560.18300492579879263No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC13440.1689276238142701No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12980.16314587478491266No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA12860.1616375924294281No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA12710.15975223948507242No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC12430.1562329139889418No Hit
GCCTACATGGAGCTGCGCAGCCTGAGATCCGAGGACACGGCCGTGTATTA12370.15547877281119948No Hit
ATTATAATGATCTAGTGCCCTCAGGGGTCTCTGACCGATTCTCTGGCTCC12140.15258789829652078No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12090.15195944731506889No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT11980.1505768551558747No Hit
GTGTATTACTGTGCGGCATGGTCGTGTGACTCCGGCGACTACCAGTGTCA11690.1469318394634537No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11590.14567493750054988No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11430.14366389435990382No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10980.13800783552683674No Hit
TTTCTGTGCAGCGTGGGATGACAGCCTGGGTGGCCGGGTGTTCGGCGGAG10960.137756455134256No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA10810.13587110218990028No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT10670.13411143944183498No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG10560.1327288472826408No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT10480.13172332571231776No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10210.12832969041247752No Hit
GTCTGGGCCTGAGGTGAAGAAGCCTGGGACCTCAGTGAAGGTCTCCTGCA10200.12820400021618714No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC10130.12732416884215447No Hit
GTTCCTCACTATGGACTGGATTTGGAGGATCGTCTTCTTGGTGGGAGCAG9580.1204112080461836No Hit
GACTATTTCTGTGCAGCGTGGGATGACAGCCTGGGTGGCCGGGTGTTCGG9280.11664050215747222No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGGCCACCCAGG9110.11450376882053577No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9060.11387531783908388No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA8830.11098444332440513No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8810.11073306293182439No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGGCCACCCAGGC8770.11023030214666286No Hit
GTCTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC8660.10884770998746869No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA8590.10796787861343603No Hit
GAAATAGTCAGCCTCATCCTCAGTCTGGAGCCCACTGATGGCCAGGGAGG8540.10733942763198412No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG8490.10671097665053224No Hit
GATCTAGTGCCCTCAGGGGTCTCTGACCGATTCTCTGGCTCCAAGTCTGG8480.10658528645424185No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8420.10583114527649957No Hit
CCTCAGGGGTCTCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCC8380.10532838449133806No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8330.10469993350988617No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA8150.10243750997665933No Hit
GTATTACTGTGCGGCATGGTCGTGTGACTCCGGCGACTACCAGTGTCAAA8090.10168336879891705No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC8080.10155767860262667No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCGGTGT8070.1014319884063363No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCCCCTCTCTTCCTCACCCTCA8060.10130629821004593No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG7960.10004939624714212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9950.060.7310561
GGTATCA4550.060.0795751
ATCAACG10300.057.9397283
TATCAAC11000.054.9166872
TCAACGC11150.053.5227974
CAACGCA12350.048.8982355
AACGCAG13650.044.2217526
CGCAGAG13700.043.6292728
GTACATG21850.039.178711
ACATGGG21050.038.9393163
ACGCAGA15400.038.8130537
GCAGAGT16700.035.3582239
TATTGAG13100.034.0252465
CGTAGTT1153.3154152E-431.2672632
CATGGGA13100.030.1874894
CATGGGG11200.029.5308154
CCTATAC1504.6534755E-528.7604453
TTATTGA15050.028.6648924
TACATGG30150.028.1457252
GGTTTAT10050.027.9159981
Produced by FastQC (version 0.11.5)