FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050374
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTTG14660.1395693343513834No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12870.12252778534122132No Hit
CCCCTATATTGGTTATCTATAAAGACAATGAAAGGCCCTCAGGGACTCCT10800.10282051916745845No Hit
CTATATTGGTTATCTATAAAGACAATGAAAGGCCCTCAGGGACTCCTGAG10610.10101163966358649No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20500.070.766371
TCAACGC24950.057.13514
ATCAACG25500.055.9027753
CAACGCA25500.055.6204385
AACGCAG27100.052.6022456
TATCAAC27300.052.2616582
GGTATCA10450.050.418661
ACGCAGA30550.046.4262247
CGCAGAG31150.045.3008548
GCAGAGT39000.036.367219
GTACATG27750.029.9102211
CAGAGTA35150.029.2899829
TATACCG1000.00628517428.79845
TACATGG28300.028.7721752
TACGGGT1555.768422E-527.8694214
TAGACCG1306.753043E-427.6907675
GCATACG1100.00988613726.245331
ACATGGG30200.025.9853123
TATACTG7100.025.3507045
CATGGGG23050.023.4260314
Produced by FastQC (version 0.11.5)