FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050374
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTTG13790.13128657030733815No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG12450.052.0367553
TCAACGC12850.050.416934
GTATCAA14300.047.381421
CAACGCA14750.043.9225465
TATCAAC15300.042.8141142
AACGCAG15900.041.651226
ACGCAGA16450.039.8210337
GGTATCA8900.039.6846281
CGCAGAG17700.036.6021238
GTACATG26900.031.0829321
TACATGG26500.030.1518612
ACATGGG26750.028.2554683
GCAGAGT23500.028.1810389
TAGACCG1050.00798192627.4225435
TTCTACG1907.563949E-626.5204832
CATGGGG22150.022.748954
GTATAGA5303.6379788E-1220.400071
AAGGCCG5850.019.6879795
ACCGACC2206.1944686E-419.6320468
CTAATAC6400.019.1207963
Produced by FastQC (version 0.11.5)