FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094640
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGGTGCCTCCATCAGCAGTAATAACTGGTGGAGTTGGGTCCGCCA34450.3147153402031718No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGACTTCAGCCTCAGGGAGA30320.2769860410728642No Hit
GTCTATTACTGTGTCAGAGATATATGGGCATACTTTGAATCGGGGAGCTT27350.24985383322370827No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT26580.24281955711466782No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26230.23962215888328584No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC24550.22427464737265218No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22840.20865307315647152No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT22730.20764817656946577No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA22700.20737411386391874No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA22490.2054556749250895No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC21570.19705108528831394No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20440.1867280567127092No Hit
GAGCTACAGTATCCCAATCACTTTCGGCCCTGGGACCAAAGTGGAGATCA19280.1761309654315574No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG18300.1671782503836878No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16790.153383760871154No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG16570.15137396769714243No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15710.14351750347146094No Hit
GTCCCCACCCTCCCTGTCTGCAGCTGTAGGAGACAGAGTCACCATCACTT15390.14059416794562596No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA14300.13063655631075055No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCCA14280.13045384784038588No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG14230.1299970766644742No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT14190.1296316597237448No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG13910.12707374113863917No Hit
ACAGTATCCCAATCACTTTCGGCCCTGGGACCAAAGTGGAGATCAAACGA13890.1268910326682745No Hit
TTCTGAGACTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTC13000.11876050573704597No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12710.11611123291675803No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12560.11474091938902288No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA12340.11273112621501133No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12320.11254841774464663No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12280.11218300080391727No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11910.10880289410217059No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11890.1086201856318059No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC11870.1084374771614412No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC11840.10816341445589418No Hit
CACTTGCACTGTCTCTGGTGCCTCCATCAGCAGTAATAACTGGTGGAGTT11810.10788935175034715No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11650.10642768398742965No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC11420.10432653657823576No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG11310.10332163999123No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG11170.1020426806986772No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC11010.10058101293575972No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10980.10030695023021266No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)