FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094640
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGGTGCCTCCATCAGCAGTAATAACTGGTGGAGTTGGGTCCGCCA31870.2911459475261273No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGACTTCAGCCTCAGGGAGA27800.2539647738069137No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT26220.23953080464810347No Hit
GTCTATTACTGTGTCAGAGATATATGGGCATACTTTGAATCGGGGAGCTT25990.23742965723890957No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25600.23386684206679823No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC21190.19357962435138493No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21180.1934882701162026No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA20940.19129576847182636No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT20420.1865453482423445No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18980.1733903383760871No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC18980.1733903383760871No Hit
GAGCTACAGTATCCCAATCACTTTCGGCCCTGGGACCAAAGTGGAGATCA17630.1610575166264708No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17590.16069209968574144No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG15890.14516187970474312No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA14750.13474749689395601No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG14310.1307279105459329No Hit
GTCCCCACCCTCCCTGTCTGCAGCTGTAGGAGACAGAGTCACCATCACTT13890.1268910326682745No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13850.12652561572754514No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13370.12214061243879265No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT13350.12195790396842797No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12920.11802967185558723No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT12540.11455821091865818No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG12030.10989914492435869No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11940.10907695680771762No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCCA11640.10633632975224731No Hit
ACAGTATCCCAATCACTTTCGGCCCTGGGACCAAAGTGGAGATCAAACGA11540.10542278740042388No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11370.10386976540232407No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11290.1031389315208653No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11150.1018599722283125No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11110.10149455528758312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9850.063.5165061
ATCAACG11250.053.6675033
TCAACGC11350.053.1946684
CAACGCA11650.051.210235
AACGCAG12800.046.5815736
TATCAAC13450.044.3608782
CGCAGAG15000.039.3535658
TACACGG12900.037.867386
ACACGGG13100.037.367947
GTACACG13100.036.7627565
CAGCGCT7350.036.2389769
GTATGTA14200.035.4497531
GAATACG1459.1950096E-734.752949
TATGTAC14150.034.03792
ACGGGGT14000.033.937319
GCAGAGT18300.031.8636469
TAGACGG15650.031.6912485
GGCAGCG7750.031.5819367
ATAGACG16250.030.9620274
GTACATG21800.030.6782071
Produced by FastQC (version 0.11.5)