FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745030
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTATGATAGTACTGGTTACGTCCCGGGCGAATGGGGCCAGGGAACCC42910.5759499617465068No Hit
TCATAGGACAGTCTCGCACAGTAATAAACGGCCGTGTCCTCAAATCTCAG38280.5138048132289975No Hit
GTAATAAACGGCCGTGTCCTCAAATCTCAGGCTGGTCAGCTCCATGTAGG25730.3453552205951438No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCGTCGGTGAAGGTCTCCTGCC21150.2838811859925104No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19330.2594526394910272No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA18850.25300994590821846No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15960.2142195616283908No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC15720.21099821483698644No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15500.20804531361153242No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14850.19932083271814557No Hit
ATCATAGGACAGTCTCGCACAGTAATAAACGGCCGTGTCCTCAAATCTCA14680.19703904540756748No Hit
GTTTATTACTGTGCGAGACTGTCCTATGATAGTACTGGTTACGTCCCGGG14650.19663637705864193No Hit
TCCCTATCTTTGGTATGCCAAACTACGCACAGAAGTTCCAGGGCAGAGTC14620.19623370870971638No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14320.19220702522046093No Hit
GCCCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG14250.19126746573963463No Hit
GCCTACATGGAGCTGACCAGCCTGAGATTTGAGGACACGGCCGTTTATTA14040.1884487872971558No Hit
GTACTATCATAGGACAGTCTCGCACAGTAATAAACGGCCGTGTCCTCAAA13670.1834825443270741No Hit
GTGCGAGACTGTCCTATGATAGTACTGGTTACGTCCCGGGCGAATGGGGC13520.18146920258244634No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13160.17663718239533976No Hit
GTCTCGCACAGTAATAAACGGCCGTGTCCTCAAATCTCAGGCTGGTCAGC12340.16563091419137485No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12080.1621411218340201No Hit
TGATAGTACTGGTTACGTCCCGGGCGAATGGGGCCAGGGAACCCTGGTCA10520.14120236768989167No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10030.13462545132410775No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9740.13073299061782748No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9650.12952498557105083No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA9530.12791431217534863No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT9510.12764586660939828No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9450.12684052991154718No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG9430.12657208434559683No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT9160.12294806920526691No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC9000.120800504677664No Hit
ACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTGCAGG8900.11945827684791217No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC8830.11851871736708589No Hit
GATTTATAGTACAAGTAAGAAACATTCCTGGACCCCTGCCCGGTTCTCAG8730.11717648953733406No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA8690.11663959840543334No Hit
CCCCTGGACAAGGACTTGAGTGGATGGGAAGGATCATCCCTATCTTTGGT8610.11556581614163189No Hit
CCATGTAGGCTGTGTTCGTGGATTTGTCGGCGGTAATCGTGACTCTGCCC8610.11556581614163189No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC8490.11395514274592969No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT8420.11301558326510341No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG8240.11059957317155014No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC8200.11006268203964942No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG8090.1085862314269224No Hit
ACATGGAGCTGACCAGCCTGAGATTTGAGGACACGGCCGTTTATTACTGT8020.10764667194609615No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT7970.10697555803122023No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA7650.1026804289760144No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT7640.1025462061930392No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)