FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745030
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTATGATAGTACTGGTTACGTCCCGGGCGAATGGGGCCAGGGAACCC41710.559843227789485No Hit
TCATAGGACAGTCTCGCACAGTAATAAACGGCCGTGTCCTCAAATCTCAG37070.4975638564890005No Hit
GTAATAAACGGCCGTGTCCTCAAATCTCAGGCTGGTCAGCTCCATGTAGG24230.3252218031488665No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCGTCGGTGAAGGTCTCCTGCC21310.2860287505201133No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21130.28361274042656No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17780.23864810812987397No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15520.2083137591774828No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC15090.20254217950954995No Hit
GTTTATTACTGTGCGAGACTGTCCTATGATAGTACTGGTTACGTCCCGGG14480.19435458974806383No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14350.19260969356938645No Hit
TCCCTATCTTTGGTATGCCAAACTACGCACAGAAGTTCCAGGGCAGAGTC14180.19032790625880838No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13960.18737500503335436No Hit
GTGCGAGACTGTCCTATGATAGTACTGGTTACGTCCCGGGCGAATGGGGC13420.1801269747526945No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13350.17918741527186824No Hit
GTACTATCATAGGACAGTCTCGCACAGTAATAAACGGCCGTGTCCTCAAA13280.17824785579104196No Hit
GCCTACATGGAGCTGACCAGCCTGAGATTTGAGGACACGGCCGTTTATTA13150.17650295961236462No Hit
GCCCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG13150.17650295961236462No Hit
ATCATAGGACAGTCTCGCACAGTAATAAACGGCCGTGTCCTCAAATCTCA12650.1697918204636055No Hit
GTCTCGCACAGTAATAAACGGCCGTGTCCTCAAATCTCAGGCTGGTCAGC12590.1689864837657544No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12370.1660335825403004No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11780.15811443834476466No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10910.1464370562259238No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10640.14281304108559387No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA10540.14147081325584204No Hit
TGATAGTACTGGTTACGTCCCGGGCGAATGGGGCCAGGGAACCCTGGTCA10180.13663879306873547No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10150.13623612471980992No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9980.13395433740923185No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT9530.12791431217534863No Hit
GATTTATAGTACAAGTAAGAAACATTCCTGGACCCCTGCCCGGTTCTCAG9420.12643786156262166No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT8930.1198609451968377No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC8800.11811604901816035No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC8750.11744493510328442No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG8720.11704226675435889No Hit
CCCCTGGACAAGGACTTGAGTGGATGGGAAGGATCATCCCTATCTTTGGT8690.11663959840543334No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG8520.11435781109485524No Hit
ACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTGCAGG8510.11422358831188006No Hit
CCATGTAGGCTGTGTTCGTGGATTTGTCGGCGGTAATCGTGACTCTGCCC8400.11274713769915305No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC8200.11006268203964942No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8130.10912312255882313No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG8070.10831778586097204No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC7990.1072440035971706No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC7980.1071097808141954No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT7840.10523066185254286No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA7830.10509643906956767No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG7650.1026804289760144No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA7510.10080131001436184No Hit
ACATGGAGCTGACCAGCCTGAGATTTGAGGACACGGCCGTTTATTACTGT7470.10026441888246111No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGCT5250.042.5087559
GGCAGCG6300.039.9974827
TAGACGT750.00153087338.4182174
TACACGG9350.035.4202276
GCAGCGC6100.035.4076088
GTATGTA10300.034.9774321
GTACACG9900.034.1980325
TATGTAC10650.033.146932
GTCTATA1801.3636054E-732.0237851
ACACGGG10350.031.9979867
GAATACG1851.7474486E-731.1310869
GTCCTAT19850.030.8541491
ACGGGGT10600.030.5620639
CTATGAT19600.030.5042614
TATGATA20100.030.4622085
ATGTACA11850.029.7882753
GATAGTA19850.029.7412018
GTATATA3151.8189894E-1229.7363721
ATAGGAC19950.029.6100143
CACGGGG11450.029.5527278
Produced by FastQC (version 0.11.5)