FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634671
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA16250.25603816780662736No Hit
GTGTATTACTGTGCGAGAGGACCAATAGAATACAGTAACTCCCCGGACGG15630.2462693269426207No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCC14200.22373796817563746No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9670.15236240508862073No Hit
TACATATTCTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGA9660.15220484313920127No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC9480.14936872804965093No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9430.1485809183025536No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT9150.14416918371880863No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9080.1430662500728724No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA8860.13959988718564423No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8460.1332974092088657No Hit
ACCTAGGACGGTGACCTTGGTTCCAGTTCCGAAGACATAAGTATAGCTGC8230.12967348437221807No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT8190.12904323657454017No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA8180.1288856746251207No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA8020.12636468343440932No Hit
GACCTAGGACGGTGACCTTGGTTCCAGTTCCGAAGACATAAGTATAGCTG7820.12321344444602006No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT7810.1230558824966006No Hit
CGTCCATACCGTCCGGGGAGTTACTGTATTCTATTGGTCCTCTCGCACAG7750.12211051080008382No Hit
GATTATTACTGCTGCTCATATGCAGGCAGCTATACTTATGTCTTCGGAAC7750.12211051080008382No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT7730.12179538690124489No Hit
ATTCTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGACGACG7690.12116513910356705No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG7460.1175412142669194No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG7440.11722609036808047No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC7180.11312947968317444No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC7160.11281435578433552No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG7130.11234166993607712No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC6990.11013580264420464No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG6880.10840262120059053No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG6810.1072996875546543No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA6670.10509382026278181No Hit
GGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAA6490.10225770517323149No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7200.063.008241
GGTATCA3300.052.3704831
TATCAAC8550.052.217352
ATCAACG8550.051.3751373
TCAACGC8550.051.3751374
CAACGCA8850.049.633615
GAATACG1203.8526196E-947.991159
AACGCAG9550.045.9810456
CGCAGAG9900.044.3554578
GTACATG15500.039.4890331
GTGCTAG2900.037.246251
ACATGGG16500.036.222923
TACATGG16750.036.1121862
GCAGAGT12000.035.9933629
CATGGGG12100.034.516914
CTAGAGA3153.8198777E-1127.4321584
CTAGACC1607.162416E-526.9950229
ATGGGGC5000.025.923395
GCTAGAG3409.822543E-1125.4150893
CAGCGCT3701.0913936E-1125.2926339
Produced by FastQC (version 0.11.5)