FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences839772
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCAGTGATGGCCAGGGAGG36230.43142662532211123No Hit
GTTATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGACTCCAAG32900.3917730050537527No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTGGGTGTTCGG31540.37557813311232097No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC27550.3280652367547382No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC26690.31782436185059754No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT26340.31365656392449376No Hit
GACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAACACCCAACCATTCAGG22330.2659055076854194No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC19510.2323249643950977No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT18420.2193452508538032No Hit
GATTATAACTATAGAAGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGTTGA17420.207437256779221No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAACACCCA17410.2073181768384752No Hit
CTTCTATAGTTATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTG17360.20672277713474607No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC16030.19088514501555184No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15850.18874170608212704No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA15550.1851693078597524No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC15100.17981071052619044No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14810.17635739224456162No Hit
GGTATCAACAGCTCCCAGGAACGGCCCCCAAACTCCTCTTCTATAGTTAT14370.17111787485174546No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14120.16814087633309993No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14000.1667119170441501No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA13620.16218687929580886No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA13320.1586144810734342No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA12720.15146968462868493No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT12430.14801636634705612No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12410.14777820646556444No Hit
GTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC12240.1457538474728855No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12090.14396764836169818No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC12010.1430150088357316No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11900.14170512948752756No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11900.14170512948752756No Hit
ACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAACACCCAACCATTCAGGC11870.1413478896652901No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG11790.14039525013932352No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT11130.13253597405009931No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA10890.1296780554721996No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCT10890.1296780554721996No Hit
CTATAGAAGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGTTGATACCAGTT10640.12670105695355405No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10510.1251530177238584No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGCCCAG10310.12277141890894194No Hit
GCATGGGATGACAGCCTGAATGGTTGGGTGTTCGGCGGAGGGACTAAGCT10050.1196753404495506No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC9820.1169365018123967No Hit
TCCTCAGACTGGAGCCCAGTGATGGCCAGGGAGGCTGACGTGCCAGACTT9750.11610294222717595No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG9510.11324502364927623No Hit
CCCAGGAACGGCCCCCAAACTCCTCTTCTATAGTTATAATCAGCGGCCCT9270.11038710507137652No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGG9190.10943446554540995No Hit
TTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTGGGTGTTCGGCG9180.10931538560466413No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG9090.10824366613795174No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9010.10729102661198517No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG8970.10681470684900188No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8920.10621930714527275No Hit
TCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCA8860.10550482750079783No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG8610.1025278289821523No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCC8540.10169426939693155No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGA8450.10062254993021916No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)