FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences839772
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCAGTGATGGCCAGGGAGG32780.3903440457648028No Hit
GTTATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGACTCCAAG29730.3540246638373273No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTGGGTGTTCGG29620.35271478448912325No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC27730.330208675688163No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC26820.3193724010802932No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT25030.2980570916867912No Hit
GACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAACACCCAACCATTCAGG20390.24280399918073003No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC18020.21458205322397034No Hit
GATTATAACTATAGAAGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGTTGA17960.2138675735794954No Hit
CTTCTATAGTTATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTG17890.21303401399427463No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16900.20124509986043831No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAACACCCA16870.20088786003820083No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT16780.19981614057148844No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC15200.18100150993364866No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC14590.17373763354815355No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA14550.17326131378517026No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13940.16599743739967515No Hit
GGTATCAACAGCTCCCAGGAACGGCCCCCAAACTCCTCTTCTATAGTTAT13520.16099607988835066No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA13280.15813816131045094No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12900.1536131235621097No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA12580.14980256545824344No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA12400.14765912652481863No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11960.1424196091320025No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11720.13956169055410278No Hit
GTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC11630.1384899710873904No Hit
ACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAACACCCAACCATTCAGGC11490.13682285191694887No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11330.13491757286501574No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11040.13146425458338692No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10930.1301543752351829No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT10710.12753461653877482No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10700.12741553659802898No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC10570.1258674973683333No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT10280.12241417908670447No Hit
CTATAGAAGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGTTGATACCAGTT10260.12217601920521284No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCT9860.11741282157537998No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG9680.11526938264195519No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA9480.11288778382703878No Hit
GCATGGGATGACAGCCTGAATGGTTGGGTGTTCGGCGGAGGGACTAAGCT9410.11205422424181802No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC9030.1075291864934768No Hit
TCCTCAGACTGGAGCCCAGTGATGGCCAGGGAGGCTGACGTGCCAGACTT8890.10586206732303528No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG8780.10455218797483126No Hit
TTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTGGGTGTTCGGCG8750.1041949481525938No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGA8740.10407586821184797No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG8720.10383770833035633No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC11250.061.4674264
CAACGCA12250.055.8616565
AACGCAG13600.050.818976
CGCAGAG14550.048.4904948
ACGCAGA16700.041.816617
ATCAACG16750.040.8540533
GCAGAGT19250.035.5292789
TACATGG22150.034.4713632
GTACATG22800.033.8045541
ACATGGG23150.033.2934723
CCTATAC2507.6397555E-1131.6941413
TATTGAG13650.031.662485
GTATAGG3101.8189894E-1230.2070261
GTCTAGA1753.9687493E-628.8128551
GTACACG4550.028.4962335
TACACCG1306.732126E-427.7046685
TTATTGA15700.027.5282064
GTCTAGC1358.393059E-426.678571
TGTAACG1100.009980720526.1935042
GGTTTAT11500.025.6810251
Produced by FastQC (version 0.11.5)