FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743575
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTAGTGACTCCATAGGTACCAGTACTAACTACTGGGCCTGGATCCG41880.5632249604949063No Hit
GTAATACATGGCCGTGTCTGCGGCGCTCACAGGCCTCAGATGCAGGGAGA35490.4772887738291363No Hit
GTACTAACTACTGGGCCTGGATCCGCCAGCCCCCAGGAAAGGCTCTGGAG25550.3436102612379383No Hit
ATGTATTACTGTGCGAGACATGATCGGCCAGAAACGACGTACTGGTTCGA25340.34078606730995525No Hit
CCTTAGAAGTGTGCACTGGTACCAGCGGAAGCCAGGCCAGGCCCCTGTGT21400.2877988098039875No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20770.2793262280200383No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAC19850.2669535689069697No Hit
ACTTACTACAATCCGTCCCTCAATAGTCGAGTCACCATGTCCGTTGACAC19590.2634569478532764No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT18950.2548498806441852No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18810.2529670846921965No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18440.24799112396194065No Hit
GTTTATTACACTGGGATTACTTACTACAATCCGTCCCTCAATAGTCGAGT18350.24678075513566217No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC18290.2459738425848099No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG18220.24503244460881554No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC17230.23171838751975254No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA16970.2282217664660592No Hit
GTATTACTGTGCGAGACATGATCGGCCAGAAACGACGTACTGGTTCGACC16750.22526308711293414No Hit
CACCTGCACTGTCTCTAGTGACTCCATAGGTACCAGTACTAACTACTGGG16250.21853881585583163No Hit
CAGTAATACATGGCCGTGTCTGCGGCGCTCACAGGCCTCAGATGCAGGGA15670.2107386611975927No Hit
CCTATGTGTTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACG15650.21046969034730859No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14880.20011431261137075No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA14620.19661769155767742No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG13900.18693474094744983No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13810.18572437212117135No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT13720.1845140032948929No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG13570.18249672191776217No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13030.17523450896009146No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA12740.17133443163097198No Hit
GTGTTGGTCATCTATTATGATAGCGACCGGCCCTCAGGGATCCCTGAGCG12330.16582052920014792No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC11750.15802037454190904No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT11500.15465823891335778No Hit
TTATTACACTGGGATTACTTACTACAATCCGTCCCTCAATAGTCGAGTCA11480.15438926806307368No Hit
CCATTACCTGTGAGGGAAACAACCTTAGAAGTGTGCACTGGTACCAGCGG11160.15008573445852808No Hit
ATACATGGCCGTGTCTGCGGCGCTCACAGGCCTCAGATGCAGGGAGAGCC11010.1480684530813973No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10890.14645462797969272No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC10740.14443734660256194No Hit
CTCCATAGGTACCAGTACTAACTACTGGGCCTGGATCCGCCAGCCCCCAG10520.14147866724943683No Hit
GCCATGTATTACTGTGCGAGACATGATCGGCCAGAAACGACGTACTGGTT10520.14147866724943683No Hit
GTGTTCCCTGGCCCCAGGGGTCGAACCAGTACGTCGTTTCTGGCCGATCA10370.13946138587230608No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA10370.13946138587230608No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA10290.13838550247116968No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA10250.1378475607706015No Hit
GTAATAAACACTCCCAATCCACTCCAGAGCCTTTCCTGGGGGCTGGCGGA10250.1378475607706015No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10210.13730961907003328No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10200.13717513364489123No Hit
GTGTTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACGGCCAC10180.13690616279460716No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA10150.13650270651918098No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA10080.13556130854318663No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC9820.13206468748949332No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA9790.13166123121406717No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG9780.13152674578892512No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA9770.13139226036378307No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGTTGACTCAGCCAC9770.13139226036378307No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9770.13139226036378307No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG9740.13098880408835692No Hit
TCTATTATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGC9520.12803012473523182No Hit
GTCCCAAATGCACCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGG9520.12803012473523182No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC9420.1266852704838113No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGGACTGGCT9340.1256093870826749No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC9330.12547490165753286No Hit
CTATTATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCT9270.12466798910668057No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAATTCTGGGAACACGG9230.12413004740611236No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT9200.12372659113068622No Hit
GGCTCTGTGACCTCCTATGTGTTGACTCAGCCACCCTCAGTGTCAGTGGC9190.12359210570554416No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC9130.12278519315469187No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG9110.12251622230440777No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT9060.12184379517869752No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC9020.12130585347812932No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG8970.12063342635241907No Hit
GGATTGGGAGTGTTTATTACACTGGGATTACTTACTACAATCCGTCCCTC8950.12036445550213497No Hit
CTACAATCCGTCCCTCAATAGTCGAGTCACCATGTCCGTTGACACGTCCA8880.11942305752614062No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8760.117809232424436No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA8760.117809232424436No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8680.11673334902329958No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACACGATCACGA8680.11673334902329958No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8520.11458158222102678No Hit
GTAGTAAGTAATCCCAGTGTAATAAACACTCCCAATCCACTCCAGAGCCT8500.1143126113707427No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC8490.11417812594560064No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC8380.1126987862690381No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8370.11256430084389604No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8270.11121944659247553No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT8240.11081599031704939No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG8170.10987459234105504No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT8140.10947113606562889No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC8120.10920216521534479No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA8100.10893319436506069No Hit
CCCCTGTGTTGGTCATCTATTATGATAGCGACCGGCCCTCAGGGATCCCT7950.10691591298792992No Hit
GTCCCTCACCTGCACTGTCTCTAGTGACTCCATAGGTACCAGTACTAACT7930.10664694213764582No Hit
CTATTATCCCACACCTGACAGAAATAGTCGGCCTCATCCCCGGCCTCGAC7930.10664694213764582No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACACGATCACGAC7870.10584002958679353No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC7770.10449517533537302No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC7770.10449517533537302No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT7770.10449517533537302No Hit
GTATCAACGCAGAGTACATGGGGCTTTCTGAGAGTCATGGATCTCATGTG7730.10395723363480483No Hit
GTATCAACGCAGAGTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGT7690.10341929193423663No Hit
CGTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTG7660.10301583565881048No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA7650.10288135023366843No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA7650.10288135023366843No Hit
TCCAACAACCGGCTCTCCCTGCATCTGAGGCCTGTGAGCGCCGCAGACAC7630.10261237938338431No Hit
CTTCTAAGGTTGTTTCCCTCACAGGTAATGGTGGCCGTCTTTCCTGGGGC7630.10261237938338431No Hit
GCACAGGCTCTGTGACCTCCTATGTGTTGACTCAGCCACCCTCAGTGTCA7620.10247789395824228No Hit
GATTGGGAGTGTTTATTACACTGGGATTACTTACTACAATCCGTCCCTCA7580.10193995225767408No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA7570.10180546683253203No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)