FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1172013
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT34000.29009917125492635No Hit
ATCTAGAAGGGCCACTGACATCTCAGACAGGTTCAGTGGCAGTGGGTCTG26680.227642526149454No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21500.1834450641759093No Hit
GTATTACTGTCAGCGAGGGGACAGTTTTGGCCAGGGGACGTACCTGGAAA21290.18165327517698182No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC19740.16842816589918372No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19280.1645032947586759No Hit
GTCTGGGACAGACTTCACTCTAACCATCGACAGACTGGAGCCAGAAGATT16110.13745581320343717No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15760.1344694982052247No Hit
CATCTATGGTGTATCTAGAAGGGCCACTGACATCTCAGACAGGTTCAGTG15690.1338722352055822No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14910.12721701892385154No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14700.12542522992492403No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13660.11655160821594983No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG13590.11595434521630733No Hit
CAGTAATACATCGCAAAATCTTCTGGCTCCAGTCTGTCGATGGTTAGAGT12920.11023768507687202No Hit
TCTATGGTGTATCTAGAAGGGCCACTGACATCTCAGACAGGTTCAGTGGC12350.10537425779406885No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11780.10051083051126565No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)