FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1172013
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT27660.23600420814444892No Hit
ATCTAGAAGGGCCACTGACATCTCAGACAGGTTCAGTGGCAGTGGGTCTG22900.1953903241687592No Hit
GTATTACTGTCAGCGAGGGGACAGTTTTGGCCAGGGGACGTACCTGGAAA18770.16015180718985197No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16460.14044212820164964No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16090.13728516663211074No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC15860.13532273106185683No Hit
GTCTGGGACAGACTTCACTCTAACCATCGACAGACTGGAGCCAGAAGATT13770.11749016435824518No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12840.10955509879156629No Hit
CATCTATGGTGTATCTAGAAGGGCCACTGACATCTCAGACAGGTTCAGTG12750.10878718922059738No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12640.10784863307830203No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA12200.10409440850912062No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG16400.047.8413243
TCAACGC17500.045.245484
TATCAAC19900.040.150452
GTATCAA19950.040.0994381
CAACGCA20150.040.0095375
AACGCAG20350.039.970046
CGCAGAG21600.036.9904678
ACGCAGA22200.036.6392027
GGTATCA11050.031.9589121
GTACATG31200.031.4153921
TACATGG31500.029.2496032
GCAGAGT27400.029.1603669
ACATGGG30950.027.2110793
GAATACG4050.026.6597949
TACGCTA1400.001042314225.7076595
TTACCGT1400.001042314225.7076594
GTTAGGA16700.023.3042681
TTACACG2252.8032446E-522.3942264
AGTTACC25450.022.3439469
TAGGACC16500.022.2488123
Produced by FastQC (version 0.11.5)