FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933178
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTACTCGTCGAACTTTTGGCCAGGGGACCAAGCTGGAGATCA40800.4372156223142852No Hit
GTATAATATTGCTGACAATAATAAACTGCCACATCTTCAGCCTGCAGGCT33050.35416608621291973No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT24770.2654370334491383No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17290.1852808360248527No Hit
GTTTTATACACCTCCAACAATAAGAACTTCTTAGCTTGGTACCAGCACAA15510.1662062328944746No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13010.13941605995855025No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12920.13845161373285697No Hit
TTATTATTGTCAGCAATATTATACTACTCGTCGAACTTTTGGCCAGGGGA12470.13362938260439058No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11860.12709258040802504No Hit
ACTTAGGAGGCTGTCCTGGTTTGTGCTGGTACCAAGCTAAGAAGTTCTTA11500.12323479550525195No Hit
GTAATACACAGCCGTGTCCTCGGGAGTCACAGAGTTCAGTTGCAGGGAGA11030.11819824299329816No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAA10820.11594786846668052No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10110.108339459352878No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT9970.10683920966846625No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC14200.040.557184
ATCAACG14400.040.4938053
ATACCGC750.001535797438.394136
GTATCAA16400.037.7725871
GAATACG3500.037.0229079
CAACGCA16000.036.4444275
TATCAAC16800.035.565992
AACGCAG17450.033.003556
CTACTCG23050.032.7932479
TATACTA26150.032.7598085
CGCAGAG17450.032.5910078
GTATAAT23400.031.7061461
ATACTAC25150.031.26
TTATACT27250.031.1732144
ACGCAGA19150.030.4496547
ACTACTC25550.030.4297898
ATTATAC27900.030.188933
GTGCTAG6250.029.965081
TACTACT27250.029.0597767
TATAATA26300.027.3722382
Produced by FastQC (version 0.11.5)