FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250270
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGGTTACTCCATCAGCAGTGGTTATTACTGGGGCTGGATCCGGCA55560.44438401305318054No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA53560.4283874683068457No Hit
TTGTAGTAGTGGTCGTTGCGACCAGACCTGGGTCGACCCCTGGGGCCAGG43550.34832476185144007No Hit
CTACTACAACGTTGAAATAGTGTCGCACAGTAATACACGGCCGTGTCTGC41300.3303286490118134No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT35780.2861781855119294No Hit
GTTTTATACAGCTCCGACAATCAAAACTACTTGGCTTGGTACCAGCAGAA35680.28537835827461266No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA32760.2620234029449639No Hit
GTGTATTACTGTGCGACACTATTTCAACGTTGTAGTAGTGGTCGTTGCGA32240.25786430131091687No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT31350.25074583889879787No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT30460.2436273764866789No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29670.23730874131187665No Hit
GTATTATAGTAATTTTGTCACCTTCGGCGGAGGGACCAAGGTGGAGATCA28690.2294704343861726No Hit
ACCCAGGTCTGGTCGCAACGACCACTACTACAACGTTGAAATAGTGTCGC28640.22907052076751425No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC27820.22251193742151695No Hit
GACCTGGGTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG27270.21811288761627487No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT26370.21091444248042424No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC24620.1969174658273813No Hit
GGTTATTACTGGGGCTGGATCCGGCAGCCCCCAGGGAAGGGGCTGGAGTG23850.19075879610004237No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG23680.18939908979660394No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT22950.18356035096419174No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21100.16876354707383207No Hit
CTATAATACTGCTGACAGTAATAAATTGCCACATCTTCAGCCTGCAGGCT20500.1639645836499316No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG20430.1634047045838099No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19970.15972549929215288No Hit
CACCTGCACTGTCTCTGGTTACTCCATCAGCAGTGGTTATTACTGGGGCT19970.15972549929215288No Hit
CCTTCCCAGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG19390.1550865013157158No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCCAAGTAGTTTTGA19370.15492653586825247No Hit
GTATTACTGTGCGACACTATTTCAACGTTGTAGTAGTGGTCGTTGCGACC19280.1542066913546674No Hit
TCCCTGAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGCT19260.15404672590720406No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18620.1489278315883769No Hit
TTATTACTGTCAGCAGTATTATAGTAATTTTGTCACCTTCGGCGGAGGGA18300.14636838442896333No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA18290.14628840170523166No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG18220.14572852263910996No Hit
ACTACAACCCGTCCCTGAAGAGTCGAGTCACCATATCAGTAGACACGTCC17840.14268917913730633No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA17090.1366904748574308No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT16360.1308517360250186No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16210.1296519951690435No Hit
GCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGTTCCACGACAC16090.1286922024842634No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC15950.12757244435201998No Hit
GGATTGGGAGTATGCATCATAGTGGGAGCGCCTACTACAACCCGTCCCTG15870.12693258256216655No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGG15650.12517296264006975No Hit
GTGTAGGTCTGGGTGCCGAAGTTGCTGGAGGGCACGGTCACCACGCTGCT15500.12397322178409463No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG15220.12173370551960777No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT14910.11925424108392586No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA14880.11901429291273084No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCTGGGAAGGTGTGCA14790.11829444839914578No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT14750.11797451750421908No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14610.11685475937197566No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCT14510.11605493213465892No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAA14240.11389539859390371No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAAC14060.11245570956673358No Hit
GTCCTGAGGACTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGA13980.1118158477768802No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13810.11045614147344174No Hit
GATTGGGAGTATGCATCATAGTGGGAGCGCCTACTACAACCCGTCCCTGA13790.1102961760259784No Hit
GTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC13550.10837659065641822No Hit
TCCTGGAGCAGGGCGCCAGGGGGAAGACCGATGGGCCCTTGGTGGAGGCT13420.10733681524790645No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA13370.10693690162924807No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCA13370.10693690162924807No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13360.10685691890551641No Hit
GCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAG13190.10549721260207795No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13000.10397754085117615No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAG12990.10389755812744447No Hit
ATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGAGCT12810.10245786910027435No Hit
CTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGCCGGATCCAGCCCCA12750.10197797275788431No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12750.10197797275788431No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA12680.10141809369176259No Hit
ATTTATTACTGTCAGCAGTATTATAGTAATTTTGTCACCTTCGGCGGAGG12550.1003783182832508No Hit
GTCCCAGGTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGG12530.10021835283578746No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG30500.047.442213
TATCAAC31100.046.5697562
TCAACGC31050.046.374
CAACGCA31700.045.646295
AACGCAG32350.045.17426
CGCAGAG36800.039.7115558
GTATCAA42100.038.0494271
ACCGTCC1157.499095E-637.5596968
GCAGAGT39800.036.3564649
GAATACG2650.035.3155639
CAGCGCT7550.032.419089
GTACATG37250.030.9935251
ACGCAGA47450.030.7984267
TACATGG37100.030.1041342
ACATGGG38750.029.1673283
TCTACAC5450.029.0599523
CATGGGG24800.028.4474354
AGCGCTG7850.028.4289445
ACCGTGA1555.77288E-527.8668758
TAGACTG3153.8198777E-1127.4245415
Produced by FastQC (version 0.11.5)