FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250270
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGGTTACTCCATCAGCAGTGGTTATTACTGGGGCTGGATCCGGCA47570.38047781679157305No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA44960.35960232589760616No Hit
TTGTAGTAGTGGTCGTTGCGACCAGACCTGGGTCGACCCCTGGGGCCAGG33890.271061450726643No Hit
CTACTACAACGTTGAAATAGTGTCGCACAGTAATACACGGCCGTGTCTGC33750.2699416925943996No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT30250.24194773928831373No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT27930.22339174738256537No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA27740.22187207563166356No Hit
GTGTATTACTGTGCGACACTATTTCAACGTTGTAGTAGTGGTCGTTGCGA27520.22011245570956675No Hit
GTTTTATACAGCTCCGACAATCAAAACTACTTGGCTTGGTACCAGCAGAA27490.2198725075383717No Hit
GTATTATAGTAATTTTGTCACCTTCGGCGGAGGGACCAAGGTGGAGATCA26260.21003463251937582No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT25840.2066753581226455No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24050.19235845057467585No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC23950.19155862333735912No Hit
CTATAATACTGCTGACAGTAATAAATTGCCACATCTTCAGCCTGCAGGCT23460.1876394698745071No Hit
ACCCAGGTCTGGTCGCAACGACCACTACTACAACGTTGAAATAGTGTCGC23140.18508002271509352No Hit
GACCTGGGTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG22690.18148080014716822No Hit
GGTTATTACTGGGGCTGGATCCGGCAGCCCCCAGGGAAGGGGCTGGAGTG22470.1797211802250714No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC20760.16604413446695512No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT20050.1603653610820063No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG18910.15124733057659545No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCCAAGTAGTTTTGA17950.14356898909835475No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT17710.1416494037287946No Hit
CACCTGCACTGTCTCTGGTTACTCCATCAGCAGTGGTTATTACTGGGGCT17580.14060962832028284No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17250.13797019843713756No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16520.1321314596047254No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG16520.1321314596047254No Hit
GTATTACTGTGCGACACTATTTCAACGTTGTAGTAGTGGTCGTTGCGACC16180.12941204699784847No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15930.12741247890455662No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA15750.12597278987738647No Hit
TCCCTGAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAACCAGCT15340.12269349820438785No Hit
CCTTCCCAGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG14940.11949418925512088No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGG14660.11725467299063402No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA14540.11629488030585394No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT14500.11597494941092724No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA14280.11421532948883042No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG13720.10973629695985668No Hit
GTCCTGAGGACTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGA13620.10893646972253994No Hit
TTATTACTGTCAGCAGTATTATAGTAATTTTGTCACCTTCGGCGGAGGGA13550.10837659065641822No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13260.10605709166819967No Hit
ACTACAACCCGTCCCTGAAGAGTCGAGTCACCATATCAGTAGACACGTCC13230.10581714349700465No Hit
GTGTAGGTCTGGGTGCCGAAGTTGCTGGAGGGCACGGTCACCACGCTGCT13130.10501731625968791No Hit
GCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGTTCCACGACAC12940.10349764450878611No Hit
GGATTGGGAGTATGCATCATAGTGGGAGCGCCTACTACAACCCGTCCCTG12920.10333767906132275No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA12840.10269781727146937No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12680.10141809369176259No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGCT8550.047.9938439
TCAACGC20650.045.6696854
ATCAACG20950.045.01573
ATACCGC1002.8705654E-643.194466
TATCAAC22350.042.1959272
CAACGCA22100.042.021765
AACGCAG22600.041.4106146
CGCAGAG24750.036.9407168
GCAGCGC11800.035.9953848
CAGGGCA12850.034.7348444
GCAGAGT28300.032.052429
TTACTCC29150.030.623869
GTATCAA33950.030.343691
GTTACTC28950.030.3380788
GGTTACT28750.030.2987217
GTACATG36350.030.1239091
TGGTTAC29000.030.0375256
TACATGG36100.029.3147982
ATACGTG1000.006287858428.7963078
CTGGTTA30950.028.6102225
Produced by FastQC (version 0.11.5)