FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences765342
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA47230.6171097365622167No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT36670.47913220494889863No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT27750.36258300210886113No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG21400.2796135583830497No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT12420.1622803922952092No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11770.15378745711067732No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11640.15208887007377095No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC11600.15156622790856897No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11510.15039028303686458No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10620.1387614948611209No Hit
ATATATGGCCGTGTCCTCGGGTCTCAGGCTGTCCATCCGCAGGATCACTG10460.13667092620031307No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10040.13118318346569247No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG9780.1277860093918797No Hit
GCCATATATTATTGTTCTTCTTGTCGTGACGGCACCATCTCTGACTTCTG8850.11563457905093409No Hit
CTCCTGTACAGCCTCTGGACTCACCCTCAGTGACCACCACGTGGACTGGG8070.10544305682949583No Hit
GATCTATGGTGCATCCAATTTTCAAAGTGGCGTCCCATCACGGTTCAGTG7960.10400579087519043No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCCG900.00375465731.9966585
ACTATAC3652.401066E-1023.6687623
GCCATAT9450.022.1522711
CTATACT5200.022.1515354
AATAGGG8200.021.9632782
ATAGGGG8600.021.7651713
CAATAGG9000.021.6557561
TATTAGG4359.094947E-1121.5290622
TAGGGGT8400.021.4263344
TATACTG5101.8189894E-1221.174265
GTACTGG4109.895302E-1021.1275671
GTAGGTC8550.021.0504369
GTATTAG3457.8505764E-820.9234331
TACATCG2455.3961172E-520.569285
CATGGGG12700.020.407324
GTAGACC1800.004484842519.9979114
GGCCGTG9050.019.8874267
CCTATAC2908.761297E-619.8599973
GTCCTAA2557.190831E-519.8157231
GTCCTAG3301.1856009E-619.687051
Produced by FastQC (version 0.11.5)