FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences803119
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCCTCGGCTCTCAGGCTGTTCAGTTGCAAAAACA16120.200717452830776No Hit
GTTTATTACTGTGCGAATACTTTTTCATGTTCTGGTGGTCGCTGTTATCC13990.17419585391455064No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11170.13908275112405508No Hit
ATACTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGGGACAACG8950.11144052126770754No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA8560.10658445386051132No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8340.10384513378465707No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22950.044.644981
CGCTTGC1505.7480065E-1043.1960229
TCAACGC24850.039.1110844
TTACACG1851.03682396E-1038.9153334
ATCAACG25800.037.9499933
CAACGCA25500.037.831815
TAGTACG1553.68982E-837.1578674
AACGCAG27050.035.6639986
ACGCTTG1854.476533E-935.02388
TATCAAC28500.034.8881842
CGCAGAG29300.032.9252938
ACGCAGA32350.030.0436027
GCAGAGT34250.028.1667469
CAGCCGT9400.027.5719289
GGTATCA16450.027.1952321
ACAGCCG10000.026.6375488
ACACCGG1450.001277391824.82536
GTTCCGA1450.001277391824.82536
GGACCGA1450.001277391824.82536
TAGTACC2752.1054439E-723.5614664
Produced by FastQC (version 0.11.5)