FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences803119
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCCTCGGCTCTCAGGCTGTTCAGTTGCAAAAACA14140.17606357214808765No Hit
GTTTATTACTGTGCGAATACTTTTTCATGTTCTGGTGGTCGCTGTTATCC13630.16971333015406187No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA850.002839191633.8752484
ACAGCCG9400.032.1634678
CAGCCGT9500.031.0671679
TACACGG5900.029.2819946
GTATCAA18850.028.291031
TCAACGC18650.028.1763954
GTACACG6250.027.6422025
TACATGG30100.026.3067762
ATCAACG19500.026.2098873
CAACGCA20200.026.0143455
CCATGCG1959.249621E-625.8407339
GTACATG29650.025.763781
ACATGGG29200.025.1454123
TAAACAG13800.025.0382275
AACGCAG21100.024.904736
ACACGGG7350.024.4846637
CGCAGAG21200.024.4477028
GTATGTA10050.024.3804231
TATCAAC21850.024.0498752
ACGGGGT7200.023.9949679
Produced by FastQC (version 0.11.5)