FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705459
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18220.2582715650377981No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC15960.22623568485198997No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACTTTTTGGGTG11460.16244742784484995No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9190.13026979597680377No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCAC8940.1267260039208515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8620.12218995008923268No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA8400.11907141307999473No Hit
GTAATAGTCAGCCTCATCCTCAGGCTGGAGCCCAGAGATGACCAGGGAGG8210.11637813111747104No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT8060.11425185588389969No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT7350.10418748644499538No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT7260.10291172130485259No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC7230.10248646625813831No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG26300.054.4733773
TCAACGC26800.053.4570854
CAACGCA27650.052.0741045
AACGCAG28900.050.0708776
CGCAGAG32450.044.3713078
ACGCAGA33300.043.238717
GTATCAA34500.041.8207131
GCAGAGT36750.039.375469
TATCAAC40250.035.4350132
GTGCTAG3950.034.7006031
GGTATCA23850.030.2476841
GAATACG2401.7025741E-929.9968539
CCTATAC3002.0008883E-1128.7969823
ATACCGT1050.00797570327.4256946
CAGCGCT2904.292815E-1027.3074849
CTAGGTC10450.026.8679963
GTACATG24750.026.2329921
GTGTAGA4950.026.2329921
ATAGACG1658.82694E-526.1790733
AGCATCG1658.82694E-526.1790737
Produced by FastQC (version 0.11.5)